攀茎钩藤茎枝的化学成分研究  

Chemical constituents from stems of Uncaria scandens

作　　者：王子明 杨龄 房银东 陈佳 王赟 李荣生 王扣 王飞 WANG Zi-ming;YANG Ling;FANG Yin-dong;CHEN Jia;WANG Yun;LI Rong-sheng;WANG Kou;WANG Fei(School of Phannacy and Yunnan Key Laboratory of Natural Medicine Pharmacology,Kunming Medical University,Kunming 650500,China;Tongren Polytechnic College,Tongren 554300,China;Yunnan Xili Biotechnology Co.,Ltd.,Kunming 650201,China;Yunnan Key Laboratory of Dai and Yi Medicines,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [2]铜仁职业技术学院,贵州铜仁554300 [3]云南西力生物技术股份有限公司,云南昆明650201 [4]云南中医药大学云南省傣医药与彝医药重点实验室,云南昆明650500

出　　处：《中草药》2019年第12期2802-2808,共7页Chinese Traditional and Herbal Drugs

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项(2014FB014);昆明医科大学“百名中青年学术和技术骨干”培养计划(60117190410);云南省重点实验室培养计划资助项目(2017DG006)

摘　　要：目的研究攀茎钩藤Uncaria scandens茎枝的化学成分。方法利用硅胶、Sephadex LH-20、RP18和HPLC等对其化学成分进行分离和纯化,根据波谱数据和理化性质对化合物的结构进行鉴定。利用高效液相色谱法测定了其中钩藤碱和异钩藤碱的含量。结果从攀茎钩藤茎枝的95%乙醇提取物中分离得到17个化合物,分别鉴定为5α-carboxystrictosidine(1)、3β-异二氢卡丹宾碱(2)、异长春花苷内酰胺(3)、3α-二氢卡丹宾碱(4)、帽柱叶碱(5)、恩卡林碱F(6)、3β-异二氢卡丹宾碱4-氧化物(7)、异二氢卡丹宾碱(8)、nauclefine(9)、卡丹宾碱(10)、calyxamine B(11)、绿原酸甲酯(12)、3,4,5-三甲氧基苯酚(13)、托可醌(14)、喹诺瓦酸(15)、咖啡酸甲酯(16)、金丝桃苷(17)。攀茎钩藤中钩藤碱和异钩藤碱的质量分数分别为0.72和0.38 μg/g。结论化合物7~9、11~14为首次从该属植物中分离得到;化合物1~6、16、17为首次从该植物中分离得到。攀茎钩藤中钩藤碱和异钩藤碱的含量较低,其在部分地区作为钩藤使用的合理性有待进一步验证。Objective To study the chemical constituents from the stems of Uncaria scandens. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, RP18, and HPLC. The structures were identified by spectroscopic methods and physicochemical properties. The contents of rhynchophylline and isorhynchophylline were detected by HPLC method. Results Seventeen compounds were isolated and identified from 95% ethanol extract of the stems of U. scandens and characterized as 5α-carboxystrictosidine (1), 3β-isodihydrocadambine (2), strictosamide (3), 3α-dihydrocadambine (4), mitraphylline (5), uncarine F (6), 3β-isodihydrocadambine 4-oxide (7), isodihydrocdambine (8), nauclefine (9), cadambine (10), calyxamine B (11), methyl chlorogenate (12), 3,4,5-trimethoxyphenol (13),α-tocopherylquinone (14), quinovic acid (15), methyl caffeate (16), and hyperoside (17). The contents of rhynchophylline and isorhynchophylline from U. scandens were 0.72 and 0.38 μg/g. Conclusion Compounds 7-9 and 11-14 are isolated from Uncaria genus for the first time. Compounds 1-6 and 16, 17 are isolated from U.scandens for the first time. The contents of rhynchophylline and isorhynchophylline is low and the reasonable use of them as U. scandens in some areas needs to be further verified.

关 键 词：攀茎钩藤 吲哚生物碱 3β-异二氢卡丹宾碱 异长春花苷内酰胺 绿原酸甲酯 托可醌 

分 类 号：R284[医药卫生—中药学]