沙棘叶中鞣质类成分及其抗炎和抗肥胖活性研究  

Inhibitory effects of tannins from leaves of Hippophae rhamnoids and their anti-inflammatory and anti-obesity effects

作　　者：杨志刚 郑文惠 张凯雪 孙丽丽 包芳 张柳 矢作忠弘 北中进[2] 松崎桂一 YANG Zhi-gang;ZHENG Wen-hui;ZHANG Kai-xue;SUN Li-li;BAO Fang;ZHANG Liu;Yahagi Tadahiro;Kitanaka Susumu;Matsuzaki Keiichi(School of Phamiacy,Lanzhou University,Lanzhou 730000,China;College of Pharmacy,Nihon University,Funabashi 274-8555,Japan)

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]日本大学药学部,日本船桥274-8555

出　　处：《中草药》2019年第12期2809-2816,共8页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划政府间国际科技创新合作重点专项(2016YFE0129000);甘肃省中医药管理局科研课题(GZK-2015-21);兰州大学中央高校基本科研业务费专项资金资助(lzujbky-2017-k26)

摘　　要：目的研究沙棘Hippophae rhamnoides叶的化学成分及其抗炎和抗肥胖活性。方法采用硅胶、ODS、Sephadex LH-20、MCI CHP20柱色谱和半制备液相等方法进行分离纯化,通过波谱分析鉴定化合物的结构。对分离得到的化合物进行体外抑制RAW264.7巨噬细胞一氧化氮生成的抗炎活性和抑制3T3-L1脂肪细胞三酰甘油蓄积的抗肥胖活性评价。结果从沙棘叶中分离得到18个鞣质类化合物,分别鉴定为1,2,6-三-O-没食子酰基-β-D-吡喃葡萄糖(1)、1,3,6-三-O-没食子酰基-β-D-吡喃葡萄糖(2)、1,4,6-三-O-没食子酰基-β-D-吡喃葡萄糖(3)、1,3,4,6-四-O-没食子酰基-β-D-吡喃葡萄糖(4)、1,2,3,6-四-O-没食子酰基-β-D-吡喃葡萄糖(5)、1,2,3,4,6-五-O-没食子酰基-β-D-吡喃葡萄糖(6)、1-O-没食子酰基-4,6-(S)-六羟基联苯甲酰基-β-D-吡喃型葡萄糖(7)、1-O-没食子酰基-2,3-(S)-六羟基联苯甲酰基-β-D-吡喃型葡萄糖(8)、1,3-二-O-没食子酰基-4,6-(S)-六羟基联苯甲酰基-β-D-吡喃型葡萄糖(9)、1,6-二-O-没食子酰基-2,3-(S)-六羟基联苯甲酰基-β-D-吡喃型葡萄糖(10)、木麻黄鞣亭(11)、1,2,3-三-O-没食子酰基-4,6-(S)-六羟基联苯二甲酰基-β-D-吡喃型葡萄糖(12)、1,4,6-三-O-没食子酰基-2,3-(S)-六羟基联苯二甲酰基-β-D-吡喃型葡萄糖(13)、hippophaenin B(14)、pedunculagin(15)、木麻黄鞣宁(16)、鞣花酸(17)和松醇(18)。结论化合物2、3、5、6、8、10、12和13为首次从该属植物中分离得到,沙棘叶中的鞣质类化合物具有较强的抗炎活性和抗肥胖活性。Objective To study the anti-inflammatory and anti-obesity constituents from the leaves of Hippophae rhamnoids. Methods Several open-column chromatographic techniques and semi-preparative HPLC were used to separate and purify the compounds in H. rhamnoids. The structures of isolated compounds were elucidated by the spectroscopic analysis. Their inhibitory effects on nitric oxide production in RAW264.7 cells, and triglyceride accumulation in 3T3-L1 cells were examined. Results Eighteen tannins and other compounds were isolated and identified as 1,2,6-tri-O-galloyl-β-D-glucopyranose (1), 1,3,6-tri-O-galloyl-β-D-glucopyranose (2), 1,4,6-tri-O-galloyl-β-D-glucopyranose (3), 1,3,4,6-tetra-O-galloyl-β-D-glucopyranose (4), 1,2,3,6-tetra-O-galloyl-β-D-glucopyranose (5), 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (6), 1-O-galloyl-4,6-(S)-HHDP-β-D-glucopyranose (7), 1-O-galloyl-2,3-(S)-HHDP-β-D-glucopyranose (8), 1,3-di-O-galloyl-4,6-(S)-HHDP-β-D-glucopyranose (9), 1,6-di-O-galloyl-2,3-(S)-HHDP-β-D-glucopyranose (10), casuarictin (11), 1,2,3-tri-O-galloyl-4,6-(S)-HHDP-β-D-glucopyranose (12), 1,4,6-tri-O-galloyl-2,3-(S)-HHDP-β-D-glucopyranose (13), hippophaenin B (14), pedunculagin (15), casuarinin (16), ellagic acid (17), and pinitol (18) Conclusion Tannins from the leaves of H. rhamnoides showed anti-inflammatory and anti-obesity activities. Compounds 2, 3, 5, 6, 8, 10, 12, and 13 were isolated from this genus for the first time.

关 键 词：沙棘叶 鞣质 抗炎 抗肥胖 1 3 6-三-O-没食子酰基-β-D-吡喃葡萄糖 1 2 3-三-O-没食子酰基-4 6-(S)-六羟基联苯二甲酰基-β-D-吡喃型葡萄糖 

分 类 号：R284.1[医药卫生—中药学]