丹参水溶性成分促斑马鱼血管生成及心脏保护活性的谱-效关系研究  

Spectrum-effect relationship of angiogenesis activity and cardioprotection of water-soluble components of Salvia miltiorrhiza in zebrafish

作　　者：杨龙飞[1] 赵渤年 丁晓彦[2] 孙启慧 吕婧[1] 高燕[1] YANG Long-fei;ZHAO Bo-nian;DING Xiao-yan;SUN Qi-hui;LV Jing;GAO Yan(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Key Laboratory of Chinese Medicine Quality Standard Research,Shandong Academy of Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省中医药研究院,山东省中药质量标准研究重点实验室,山东济南250014

出　　处：《中草药》2019年第12期2899-2907,共9页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划项目(2017YFC1701501);国家重点研发计划项目(2017YFC1702700);山东省重点研发计划项目(2017CXGC1306)

摘　　要：目的研究丹参水溶性成分促血管生成及心脏保护作用的谱-效关系,揭示其药效物质基础。方法采用HPLC法建立丹参水溶性成分指纹图谱,以斑马鱼建立不同药效学模型,对丹参水溶性成分促血管生成及心脏保护作用进行评价,利用灰色关联度分析方法,建立丹参水溶性成分促血管生成及心脏保护作用的谱-效关系。结果在丹参水溶性成分HPLC指纹图谱中,9种水溶性成分与各药效作用均呈正相关,且各共有峰与药效关联度趋势基本一致,其中5号峰(紫草酸)对各药效作用贡献最大。结论通过谱-效关系研究,阐明了丹参水溶性成分促血管生成及心脏保护作用的物质基础,可为其深入研究奠定基础。Objective To study the spectrum-effect relationship of angiogenesis activity and protective effect of heart injury of water-soluble components of Salvia miltiorrhiza to reveal the pharmacodynamics material basis. Methods The HPLC fingerprints of water-soluble components of S. miltiorrhiza were established to evaluate the angiogenesis activity and protective effect of heart injury of water-soluble components of S. miltiorrhiza by establishing different models of zebrafish. The spectrum-effect relationship of angiogenesis activity and protective effect of heart injury of water-soluble components of S. miltiorrhiza was established by grey correlation degree analysis. Results Nine water-soluble components in the fingerprints were positively correlated with efficacy of medicine, the association trend of common peaks and efficacy was consistent, and peak 5 (alkannic acid) played the most significant role among nine components. Conclusion Elucidation of the material basis of angiogenesis activity and protective effect of heart injury of water-soluble components of S. miltiorrhiza by researching on the spectrum-effect relationship will provide the basis for the deep research of S. miltiorrhiza.

关 键 词：丹参水溶性成分 谱-效关系 促血管生成 心脏保护 斑马鱼 灰色关联度分析 紫草酸 

分 类 号：R285.5[医药卫生—中药学]