模拟微重力对金黄色葡萄球菌基因表达及表型的影响  

Effects of Simulated Microgravity on Gene Expression and Phenotype in Staphylococcus aureus

作　　者：王言吉 王佳平[1] 刘宇[1] 赵光显 高建义[1] 韩延平 李勇枝[1] Wang Yanji;Wang Jiaping;Liu Yu;Zhao Guangxian;Gao Jianyi;Han Yanping;Li Yongzhi(Astronaut Research and Training Center,Beijing 100094,China)

机构地区：[1]中国航天员科研训练中心,北京100094 [2]军事科学院军事医学研究院微生物流行病研究所,北京100071

出　　处：《航天医学与医学工程》2019年第3期196-202,共7页Space Medicine & Medical Engineering

基　　金：“重大新药创制”科技重大专项项目(2015ZX09J15102);1226工程卫生专业重大项目(AWS16J018);国家自然科学基金项目(31401183,9143011);973计划课题(2014CB744405)

摘　　要：目的研究金黄色葡萄球菌在低剪切力模拟微重力系统(low-shear modeled microgravity,LSMMG)条件下连续培养14d的表型变化及基因表达改变。方法利用旋转细胞培养系统模拟微重力环境对金黄色葡萄球菌连续传代培养14d,对菌株进行增殖速率、耐酸性和生物膜形成的测定,评估金黄色葡萄球菌的表型变化。利用转录组测序检测模拟微重力条件下差异表达的基因,与表型作比对。结果模拟微重力导致金黄色葡萄球菌增殖速率降低,耐酸性增强,生物膜形成能力增强,耐碱性下调;共有172个显著差异表达基因(P<0.05),其中20个上调,152个下调。36个营养代谢相关差异表达基因中32个表达下降,1个与耐碱相关基因表达下调。结论模拟微重力引起金黄色葡萄球菌表型及相应的基因表达变化,其中生物膜形成能力的增强可能对航天飞行造成潜在威胁。Objective To detect the phenotypic changes and gene changes of Staphylococcus aureus under low-shear modeled microgravity(LSMMG)conditions.Methods Continuous culture of Staphylococcus aureus was performed using a rotating cell culture system to simulate the microgravity environment.The proliferation rate,acid resistance and biofilm formation of the strains were measured.The phenotypic changes of Staphylococcus aureus were evaluated.Transcriptome sequencing was used to detect the differentially expressed genes under simulated microgravity conditions and then they were compared with the phenotypes.Results Simulated microgravity resulted in a decrease in the proliferation rate of Staphylococcus aureus,an increase in the acid resistance,an enhancement in biofilm formation,and a down-regulation of alkalinity.There were a total of 172 differentially expressed genes in the simulated microgravity group(P<0.05,of which 20 were up-regulated and 152 were down-regulated),there were 36 differentially expressed genes related to nutrition metabolism,32 of which decreased in expression,and 1 was associated with decreased expression of alkali-resistant genes.Conclusion Simulated microgravity can cause the phenotype and corresponding genetic changes in Staphylococcus aureus,and the enhancement of biofilm formation may pose a potential threat to space flight.

关 键 词：模拟微重力 金黄色葡萄球菌 表型 基因 转录组测序 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]