HIF-1ɑ和MVD在卵巢子宫内膜异位症中的表达情况分析  

To analyse the expression of HIF-1ɑ and MVD in ovarian endometriosis cyst

作　　者：韩鑫[1] 孙永红[2] 陈昭日[3] 王鑫[1] HAN Xin;SUN Yonghong;CHEN Zhaori;WANG Xin(Department of Obstetrics and Gynecology,Weifang Medical University,Weifang 261053,China;Department of Pathology,the Affiliated Hospital of Weifang Medical University;Department of Gynecology,the Affiliated Hospital of Weifang Medical University)

机构地区：[1]潍坊医学院妇产科学教研室,山东潍坊261053 [2]潍坊医学院附属医院病理科 [3]潍坊医学院附属医院妇科

出　　处：《潍坊医学院学报》2019年第3期218-221,F0004,共5页Acta Academiae Medicinae Weifang

摘　　要：目的研究缺氧诱导因子-1ɑ(HIF-1ɑ)和微血管密度(MVD)在卵巢子宫内膜异位症中的表达,探讨两者在子宫内膜异位症(EMT)发病机制中的作用。方法取23例卵巢子宫内膜异位囊肿患者的异位内膜组织、14例在位内膜组织为实验组,20例子宫平滑肌瘤患者的正常子宫内膜组织为对照组,应用免疫组织化学方法检测HIF-1ɑ、MVD在异位内膜组、在位内膜组及正常内膜组中的表达,并根据CD34单克隆抗体在血管内皮细胞中的表达计算组织中的微血管密度(MVD)。结果①HIF-1ɑ、MVD在异位内膜组的表达最高,在位内膜组次之,正常内膜组最低,差异均有统计学意义(P<0.05)。②3组分泌期与增殖期表达强度比较,差异无显著性(P>0.05)。③在内异症r-AFS分期中比较,HIF-1ɑ、MVD在Ⅲ~Ⅳ期异位内膜组的表达高于Ⅰ~Ⅱ期,差异均有统计学意义(P<0.05)。④HIF-1ɑ、MVD在各组中的表达均呈显著正相关。结论 HIF-1ɑ,微血管生成在异位内膜组、在位内膜组的表达高于正常内膜组,且呈显著正相关,提示两者可能与子宫内膜异位症的发生密切相关。Objective To investigate the expression of hypoxia-inducible factor-1 alpha(HIF-1ɑ) and microvessel density(MVD) in ovarian endometriosis and to explore its role in the pathogenesis of endometriosis.Methods Twenty-three cases of ectopic endometrium and 14 cases of eutopic endometrium from 23 patients with ovarian endometriosis were used as experimental group,and 20 cases of normal endometrial tissue of uterine fibroids were used as control group.The expressions of HIF-1ɑ and MVD was detected by Immunohistochemical EnVision method in ectopic endometrial group,eutopic endometrial group and normal endometrial group,and the microvessel density(MVD) was calculated according to the expression of CD34 monoclonal antibody in vascular endothelial cells.Results The expression of HIF-1ɑ and MVD in ovarian endometriosis was the highest in the ectopic endometrium group,the second in the eutopic endometrium,and the lowest in the normal endometrium. The difference was statistically significant(P<0.05).There was no significant difference in the expression intensity between the secretory phase and the proliferative phase(P>0.05).Compared with the r-AFS staging of endometriosis,the expression of HIF-1ɑ and MVD in stage Ⅲ~Ⅳ ectopic tissues was higher than in Ⅰ~Ⅱ(P<0.05),the expression of HIF-1ɑ and MVD in each group was significantly positively correlated.Conclusion HIF-1ɑ and MVD in the ectopic endometrium group,eutopic endometrial group expression was higher than the normal endometrial group,and there was a significant positive correlation,suggesting that both may be closely related to the occurrence of endometriosis.

关 键 词：子宫内膜异位症 缺氧诱导因子-1ɑ 微血管密度 CD34 免疫组织化学 

分 类 号：R711.71[医药卫生—妇产科学]