二烯丙基三硫醚对脂多糖诱导小鼠肺炎的改善作用  

Improvement of diallyl trisulfide on pneumonia in mice induced by lipopolysaccharides

作　　者：张芳 沈绍清 杨卫丽 ZHANG Fang;SHEN Shao-qing;YANG Wei-li(College of Pharmacy, Hainan Medical University, Haikou 571199, China;Department of Pharmacy, Hainan Hospital of PLA General Hospital, Sanya 572013, China)

机构地区：[1]海南医学院药学院 [2]解放军总医院海南医院药剂科

出　　处：《现代药物与临床》2019年第6期1610-1614,共5页Drugs & Clinic

摘　　要：目的探讨二烯丙基三硫醚(DATS)对脂多糖(LPS)诱导的小鼠肺炎的改善作用,并探讨其作用机制。方法小鼠随机分为对照组、模型组及DATS 20、40、80 mg/kg预防组和DATS 20、40、80 mg/kg治疗组。通过ip LPS制备小鼠急性肺炎模型,考察DATS对血清生化指标丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、炎症因子一氧化氮(NO)、细胞白介素8(IL-8)和肿瘤坏死因子α(TNF-α)、肺组织中髓过氧化物酶(MPO)、NO、NF-κB p65的影响。结果 DATS 40、80 mg/kg预防组和DATS 40、80 mg/kg治疗组能显著降低AST、NO、IL-8和TNF-α水平(P<0.05),DATS 80 mg/kg预防组和DATS 80 mg/kg能显著降低LDH水平(P<0.05),显著升高SOD水平(P<0.05)。DATS 80 mg/kg预防组和DATS 80 mg/kg治疗组能显著升高MPO和NO水平(P<0.05),并能抑制NF-κB p65的转移。结论 DATS对LPS诱导的肺部氧化损伤和炎症反应有一定的逆转作用,与DATS抑制NF-κB核转移和MPO活性有关。Objective To investigate the improvement of diallyl trisulfide(DATS) on pneumonia in mice induced by lipopolysaccharides(LPS), and study its mechanism. Methods Mice were randomly divided into control group, model group, DATS 20, 40, and 80 mg/kg prevention group, and DATS 20, 40, and 80 mg/kg treatment group. The mice model of acute pneumonia was induced by intraperitoneal injection of LPS. The effects of DATS on ALT, AST, LDH, SOD, NO, IL-8, TNF-α, MPO, NO, and NF-κB p65 were detected. Results DATS 20, 40, and 80 mg/kg prevention group and DATS 20, 40, and 80 mg/kg treatment group could significantly decrease AST, NO, IL-8, and TNF-α levels(P < 0.05). DATS 80 mg/kg prevention group and DATS 80 mg/kg treatment group could significantly decrease LDH level(P < 0.05), and significantly increase the SOD level(P < 0.05). DATS 80 mg/kg prevention group and DATS 80 mg/kg treatment group could significantly increase the MPO and NO level(P < 0.05), and can inhibit the transfer of NF-κB p65. Conclusion DATS has a certain reversal effect of oxidative damage to lung and inflammatory reaction induced by lipopolysaccharides, which is related to the inhibition of DATS on nuclear migration of NF-κB and MPO.

关 键 词：二烯丙基三硫醚 脂多糖 急性肺炎 髓过氧化物酶 一氧化氮 

分 类 号：R966[医药卫生—药理学]