肝细胞生长因子调节Fas/FasL系统抑制血管内皮细胞凋亡  

HGF suppressed apoptosis of vascular endothelial cells by regulating Fas/FasL pathway

作　　者：杨帆 钟武 邓璐 YANG Fan;ZHONG Wu;DENG Lu(Department of Emergency Medicine,the Affiliated Hospital of Southwest Medical University,LuZhou,SiChuan 646000,China;Department of Vascular Surgery,the Affiliated Hospital of Southwest Medical University,LuZhou,SiChuan 646000,China)

机构地区：[1]西南医科大学附属医院急诊医学部,四川泸州646000 [2]西南医科大学附属医院血管外科,四川泸州646000

出　　处：《重庆医学》2019年第A01期29-32,共4页Chongqing medicine

摘　　要：目的探讨肝细胞生长因子抑制血管内皮细胞凋亡的分子机制。方法人脐静脉内皮细胞(HUVECs)分为6组:A组:对照组;B组:肝细胞生长因子(HGF)干预组;C组:c-MetsiRNA干预组;D组:肿瘤坏死因子α(TNF-α)干预组;E组:HGF和TNF-α联合干预组;F组:c-MetsiRNA和TNF-α联合干预组。MTT比色法检测各组细胞增殖活性。Westernblot检测各组细胞凋亡相关蛋白caspase-2,-8,-9和caspase-3,-6,-7的表达,细胞免疫荧光检测Bax/Bcl-2,caspase3/Bcl-xl的表达水平。结果MTT检测显示,与对照组相比,HGF干预组细胞增殖活性增高,C-MetsiRNA干预组细胞活性下降,TNF-α诱导细胞凋亡水平升高;与TNF-α干预组相比,HGF和TNF-α联合干预组明显抑制细胞凋亡。westernblot检测显示HGF干预组、HGF和TNF-α联合干预组细胞caspase-2,-8,-9和caspase-3,-6,-7表达水平明显下降。细胞免疫荧光检测提示HGF干预组、HGF和TNF-α联合干预组细胞Bax、caspase3表达水平下降,Bcl-2、Bcl-xl表达升高。结论HGF明显抑制内皮细胞凋亡,对凋亡信号通路的调节有重要作用。Objective To investigate the molecular mechanism how HGF suppressed apoptosis signaling pathway of vascular endothelial cells. Methods The Human Umbilical Vein Endothelial Cells(HUVECs)were treated with:A:control group,B:hepatic growth factor (HGF),C:C-Met siRNA,D:TNF-α,E:combined HGF and TNF-α,F:combined C-Met siRNA and TNF-α.The activity of cells proliferation was be analyzed by MTT.The related molecules of apoptosis caspase-2,-8,-9 and caspase-3,-6,-7 were detected by western blot analysis.The levels of Bax,Bcl-2 and Bcl-xl were detected by immunofluorescence. Results MTT analysis indicated that HGF treatment induced cells proliferation and C-Met siRNA treatment decreased cells proliferation,compared to control group.Compared to TNF-α treatment group,HGF treatment and combined HGF and TNF-α suppressed apoptosis in HUVECs significantly.Western blot analysis suggested HGF treatment and combined HGF and TNF-α suppressed the expression of caspase-2,-8,-9 and caspase-3,-6,-7.Immunofluorescence analysis revealed HGF treatment and combined HGF with TNF-α suppressed Bax and caspase3,as well induced Bcl-2 and Bcl-xl levels. Conclusion HGF inhibits the apoptosis of vascular endothelial cells.It plays a critical role in apoptosis signaling pathway.

关 键 词：肝细胞生长因子 内皮细胞 凋亡 

分 类 号：R540.2[医药卫生—心血管疾病]