血管紧张素转换酶基因多态性与南京地区老年阿尔茨海默病病人认知关系的研究  

Relationship between angiotensin converting enzyme gene polymorphism and Alzheimer’s disease in elderly patients of Nanjing

作　　者：束婷婷 潘晓东 何一然 刘雅玲 孙晓萌 吕志刚 娄青林 SHU Ting-ting;LV Zhi-gang;PAN Xiao-dong;HE Yi-ran;LIU Ya-ling;SUN Xiao-meng;LOU Qing-lin(Department of Central Laboratory, Geriatric Hospital of Nanjing Medical University, Nanjing 210024, China;Department of Geriatrics, Geriatric Hospital of Nanjing Medical University, Nanjing 210024, China;Department of Endocrinology, Geriatric Hospital of Nanjing Medical University, Nanjing 210024, China)

机构地区：[1]南京医科大学附属老年医院中心实验室,江苏省南京市210024 [2]南京医科大学附属老年医院保健科,江苏省南京市210024 [3]南京医科大学附属老年医院内分泌科,江苏省南京市210024

出　　处：《实用老年医学》2019年第7期630-633,共4页Practical Geriatrics

基　　金：江苏省卫生厅保健局课题(BJ15024);国家中医临床研究基地开放课题(JD201525);江苏省省重点研发计划(社会发展)面上项目(BE2015715);江苏省基础研究计划面上研究项目(BK20161587)

摘　　要：目的研究血管紧张素转换酶(angiotensin converting enzyme,ACE)基因多态性与南京地区老年阿尔茨海默病(Alzheimer'sdisease,AD)的关系.方法研究共纳入113例无认知障碍的老年人(对照组)和149例AD病人(病例组).采集全血进行生化检查,并提取DNA,采用聚合酶链式反应(PCR)扩增检测ACEI/D基因型及频率.同时对研究对象进行神经心理学量表检测,比较2组间临床资料、生化指标、基因型分布及量表评分的差异.结果2组在年龄、受教育年限、卒中史、吸烟史、血清白蛋白、糖化血红蛋白、ACEI/D多态性方面差异均有统计学意义(P<0.05或P<0.01).病例组ACEII基因型频率高于对照组,DD基因型频率低于对照组(P<0.05).对照组神经心理学量表得分情况均优于病例组,差异有统计学意义(P<0.01).Logistic回归分析显示,受教育年限低、无吸烟史、高糖化血红蛋白、ACEII型纯合子是AD的独立危险因素.结论南京地区老年AD病人ACE基因的多态性与无认知障碍人群分布存在差异,该多态性可能对AD的发生发展产生影响.Objective To investigate the relationship between angiotensin converting enzyme(ACE) gene polymorphism and Alzheimers disease(AD) in the elderly patients in Nanjing.Methods A total of 262 individuals were enrolled in this study including 113 non-cognitive function individuals in control group and 149 patients with AD in case group.Blood samples were collected for biochemical tests,DNA extraction and polymerase chain reaction(PCR) amplification for the detection of genotype frequencies of ACE.Neuropsychological data were collected by professional neurologists.The biochemical indexes,genotype distribution and the scores of cognitive scales between the two groups were compared.Results There were significant differences between the two groups in age,during of education,history of stroke,history of smoking,level of albumin,HbA1 c and I/D polymorphism of ACE gene(P<0.05 or P<0.01).The frequency of II genotype of ACE was higher,and the frequency of DD genotype of ACE was lower in case group than that in control group(P<0.05).Logistic regression analysis showed that low education years,no smoking history,high levels of HbA1 c and II genotype of ACE were independent risk factors for AD.Conclusions The polymorphism of ACE gene in the elderly patients with AD in Nanjing is different from those without cognitive impairment.The frequency of genotype I/D may play an important role in the development of AD.

关 键 词：阿尔茨海默病 血管紧张素转换酶基因 基因多态性 

分 类 号：R749.16[医药卫生—神经病学与精神病学]