陆海高代回交群体抗黄萎病QTL定位  

Mapping of Verticillium Wilt Resistance QTL with Interspecific Advanced Backcross Population between Upland Cotton(Gossypium hirsutum L.) and Sea-Island Cotton(G.barbadense L.)

作　　者：郭志军[1] 赵云雷[1] 陈伟[1] 王红梅[1] 龚海燕[1] 桑晓慧[1] 崔艳丽 赵佩[1] Guo Zhijun;Zhao Yunlei;Chen Wei;Wang Hongmei;Gong Haiyan;Sang Xiaohui;Cui Yanli;Zhao Pei(Institute of Cotton Research of CAAS / State Key Laboratory of Cotton Biology,Anyang,Henan 455000,China)

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室

出　　处：《棉花学报》2019年第4期327-334,共8页Cotton Science

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP-2016-CCRI);国家重点研发计划(SQ2018YFD010081)

摘　　要：【目的】定位棉花抗黄萎病数量性状位点(Quantitative trait loci, QTL)。【方法】以海7124和TM-1配制抗感组合F1,再以鲁棉研28为轮回亲本构建的137个BC4F1家系为作图群体,筛选出多态性重复序列(Simple sequence repeat, SSR)标记,并与已发表的整合高密度遗传连锁图谱相比对,构建遗传图谱。采用复合区间作图法(Composite interval mapping,CIM)进行大田和病圃两个环境下抗黄萎病QTL定位。【结果】216个多态性SSR位点分布在26条染色体上,可覆盖棉花基因组3 380 cM(centi Morgan),标记间平均距离15.77 cM。定位到6个QTLs,分布在6条染色体上,可解释表型变异8.56%~20.26%,其中5个QTLs与前人研究结果相一致,在第1染色体上新定位到一个QTL。本研究可为分子标记辅助选择抗病育种提供帮助。【结论】定位到6个黄萎病相关QTLs,其中1个是在第1染色体上新发现的QTL。[Objective] The aim of this study was to map quantitative trait loci (QTLs) of Verticillium wilt resistance in cotton.[Method]In this study, with the cultivar Lumianyan 28(Gossypium hirsutum L.) as the recipient parent and the F1 generation obtained from the cross Hai7124 (G. barbadense) and TM-1(G. hirsutum L.) as the donor parent, an interspecific advanced backcross population was constructed. Compared with the integrated high density genetic linkage map published, simple sequence repeat(SSR) markers of polymorphism were used to construct genetic linkage map. QTLs detection was conducted by composite interval mapping method in field and disease nursery.[Result] 216 simple sequence repeated (SSR) markers of polymorphism were assigned to 26 Chromosomes with a total map distance of 3 380 cM and an average distance of about 15.77 cM between two adjacent markers. Six QTLs located on 6 chromosomes had been detected and could explain phenotypic variance with 8.56%~20.26%. Five of Six QTLs were consistent with the previous study. Moreover, one novel QTL which located on Chromosome 1 was detected in this study. These results maybe helpful for the marker-assisted development of new cultivars which were resistant to Verticillium wilt.[Conclusion] Six QTLs were detected, one of them is a new QTL on chromosome 1.

关 键 词：棉花 黄萎病 QTL定位 回交群体 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]