消肿止痛液消肿、促溃疡面愈合、镇痛的有效部位筛选  

Screening of Active Parts in Xiaozhong Zhitong Lotion for Reducing Swelling,Promoting Ulcer Healing and Analgesia

作　　者：王银洁 刘本臣 刘军 宫凯敏 赛纪虎 张久存 王宏伟 WANG Yinjie;LIU Benchen;LIU Jun;GONG Kaimin;SAI Jihu;ZHANG Jiucun;WANG Hongwei(Preparation Center,Yinchuan Hospital of TCM,Yinchuan 750001,China;School of TCM,Guangzhou University of TCM,Guangzhou 510006,China)

机构地区：[1]银川市中医医院制剂中心,银川750001 [2]广州中医药大学中药学院,广州510006

出　　处：《中国药房》2019年第13期1801-1806,共6页China Pharmacy

基　　金：宁夏回族自治区重点研发计划项目(No.2016-KJHM112);宁夏青年科技人才托举工程项目

摘　　要：目的:筛选消肿止痛液具有消肿、促溃疡面愈合、镇痛的有效部位,为其二次开发为软膏剂提供参考。方法:采用D101大孔树脂分离得到消肿止痛液的水洗脱部位和20%、40%、60%、95%乙醇洗脱部位。将120只SD大鼠随机分为正常组(n=8)和造模组(n=112);正常组大鼠不作处理,造模组大鼠采用肛周皮肤注射75%冰醋酸致肛周溃疡复制大鼠痔疮模型;将96只成模大鼠随机分为模型组[空白软膏基质0.51 g/(kg·d)],阳性组[马应龙麝香痔疮膏0.51 g/(kg·d)],消肿止痛液及其水洗脱部位和20%、40%、60%乙醇洗脱部位的高、低剂量组[按生药计给药剂量均分别为8.34、2.78 g/(kg·d),均制成含药软膏],每组8只;肛周涂抹给药,每天给药2次,连续给药7 d;观察给药第3、7天大鼠肛周局部症状以及给药第7天大鼠肛周局部黏膜组织病理形态并分别进行评分,考察各洗脱部位的消肿、促溃疡面愈合作用。分别取120只ICR小鼠随机分为模型组[空白软膏基质1.03 g/(kg·d)]、阳性组[马应龙麝香痔疮膏1.03 g/(kg·d)]和消肿止痛液及其各洗脱部位的高、低剂量组[按生药计均分别为16.65、5.55 g/(kg·d),均制成含药软膏],每组10只;经皮给药,每天给药2次,连续给药3 d;末次给药30 min后,分别通过醋酸扭体实验检测小鼠的扭体潜伏时间、15 min扭体次数以及热板致痛实验检测小鼠的痛阈值,考察各洗脱部位的全身和局部镇痛作用。结果:在消肿、促溃疡面愈合考察实验中,与正常组比较,模型组大鼠在给药第3、7天肛周局部症状评分以及在给药第7天肛周局部黏膜组织病理评分均显著升高(P<0.01);与模型组比较,阳性组和40%乙醇洗脱部位高、低剂量组大鼠上述评分均显著降低(P<0.05或P<0.01),20%乙醇洗脱部位高剂量组大鼠在给药第3、7天以及60%乙醇洗脱部位组大鼠在给药第7天肛周局部症状评分显著降低(P<0.05)。在镇痛考察实验中,与模型组比�OBJECTIVE:To screen active fractions of Xiaozhong zhitong lotion that are able to reduce swelling,promote ulcer healing and analgesia,and to provide reference for it’s secondary development of ointment preparation.METHODS:Water elution fraction and 20%,40%,60%,95%ethanol elution parts were separated by D101 macroporous resin from Xiaozhong zhitong lotion.120 SD rats were randomly divided into normal group(n=8)and modeling group(n=112).Rats in the normal group were not treated.Hemorrhoids model was established in the model group by injecting 75%glacial acetic acid into the perianal skin to induce perianal ulcer.96 model rats were randomly divided into model group[blank ointment matrix 0.51 g/(kg·d)],positive group[Mayinglong ointment,0.51 g/(kg·d)],high-dose and low-dose groups of Xiaozhong zhitong lotion and it’s water elution fraction and 20%,40%,60%ethanol elution parts[8.34,2.78 g/(kg·d)by crude drug,all make into containing drug ointment],with 8 rats in each group.The periphery of the anus was smeared with relevant medicine,twice a day,for consecutive 7 d.The local symptoms around the anus of rats 3 and 7 days after administration and the pathological morphology of the local mucosa around the anus of rats 7 days after administration were observed and scored respectively.The effects of each elution part for reducing swelling and promoting ulcer healing were investigated.120 ICR mice were randomly divided into model group[blank ointment matrix 1.03 g/(kg·d)],positive group[Mayinglong ointment 1.03 g/(kg·d)],high-dose and low-dose groups of Xiaozhong zhitong lotion and it’s each elution part[16.65,5.55 g/(kg·d)by crude drug,all make into containing drug ointment],with 10 mice in each group.Transdermal administration,twice a day,for consecutive 7 d.After 30 min of last administration,the latency time and 15 min writhing times of mice were detected by designing acetic acid writhing test;pain threshold of mice was determined by hot-plate pain test so as to investigate systemic and local analgesic effect

关 键 词：消肿止痛液 有效部位 消肿 促溃疡面愈合 镇痛 大鼠 小鼠 

分 类 号：R285.5[医药卫生—中药学]