实验动物冰冻组织切片制备关键要点的探讨  

Discussion on key points of freezing sectioning technology of experimental animals

作　　者：张頔[1] 霍桂桃[1] 屈哲[1] 吕建军[1] 杨艳伟[1] 李琛[1] 陈旭林 高苏涛[1] 林志[1] ZHANG Di;HUO Guitao;QU Zhe;LV Jianjun;YANG Yanwei;LI Chen;CHEN Xulin;GAO Sutao;LIN Zhi(National Center for Safety Evaluation of drugs, National Institutes for Food and Drug Control, Beijing 100176, China)

机构地区：[1]中国食品药品检定研究院国家药物安全评价监测中心药物非临床安全性评价研究北京市重点实验室

出　　处：《药物评价研究》2019年第7期1359-1361,共3页Drug Evaluation Research

基　　金：国家科技重大专项(2015ZX09501007-004);重大新药创制(2015ZX09501004-002)

摘　　要：目的探讨实验动物组织冰冻切片制备的关键要点和注意事项,以提高切片质量。方法采用美国赛默飞世尔恒温式冷冻切片机,对30例SD大鼠和30例食蟹猴的皮肤、肌肉、乳腺、卵巢、子宫、胃、大肠、甲状腺、肝脏、肾脏、脾脏、淋巴结、脑、脂肪等新鲜组织进行常规冰冻切片取材、包埋、切片、染色后封片。结果镜下检查组织切面平整,细胞形态完整、清晰,染色良好。结论不同组织需要区别对待,制作优良的冰冻切片就需要对取材、组织速冻、温度选择、组织包埋、切片、固定等多个环节进行严格谨慎地操作。Objective To improve the quality of slices,we explore the key points and consideration of freezing sectioning of experiment animals.Methods A total 30 SD tats and 30 cynomolgus monkey specimens of skin,muscle,mammary gland,ovary,uterus,stomach,intestine,thyroid,liver,kidney,spleen,lymph node,brain and fat,etc.were trimmed,embedded,sectioned and staining for routine freezing sections by Thermo low temperature cryostat microtome.Results Under microscope,the sections were smooth,and the cell morphology was complete,clear and well stained.Conclusion The different tissues need to be treated differently.To make fine frozen sections,it is necessary to operate strictly and carefully,including tissues preparation,quick freezing,selection of temperature,embedding,slicing and fixing.

关 键 词：病理技术 冰冻切片 制片方法 实验动物 

分 类 号：R965[医药卫生—药理学]