菌毒清提取物对鼻咽癌细胞CNE-2Z荷瘤裸鼠治疗作用的研究  

Therapeutic Effects of Junduqing Extract on Nude Mice Bearing Nasopharyngeal Carcinoma Cell CNE-2Z

作　　者：赵伟国[1] 唐蕾[1] 张海勇 辛玲 韩艳 李运景[1] ZHAO Weiguo;TANG Lei;ZHANG Haiyong;XIN Ling;HAN Yan;LI Yunjing(Zhongshan Hospital Affiliated to Sun Yat-sen University, Zhongshan, Guangdong, 528400, China)

机构地区：[1]中山大学附属中山医院/中山市人民医院药学部

出　　处：《肿瘤药学》2019年第3期383-386,405,共5页Anti-Tumor Pharmacy

基　　金：广东省中医药局立项(2010337);中山市科技攻关计划项目(2017B1087)

摘　　要：目的 研究菌毒清提取物(菌毒清颗粒浸膏粉)对鼻咽癌细胞CNE-2Z荷瘤裸鼠的治疗作用。方法 在裸鼠右腋皮下接种鼻咽癌CNE-2Z细胞悬液0.2mL,建立移植瘤模型。造模成功后将裸鼠随机分为5组,分别为模型组、阳性对照组(绿原酸0.01g·kg^-1)、菌毒清提取物高(8.8g·kg^-1)、中(4.4g·kg^-1)、低(2.2g·kg^-1)剂量组,口服给药,每日1次,连续3周,计算每组裸鼠肿瘤体积、相对肿瘤体积、相对肿瘤增殖率、脾脏指数和胸腺指数。结果 与模型组相比,高、中、低剂量组裸鼠胸腺指数显著升高,差异均具有统计学意义(P<0.05);与模型组相比,阳性对照组、高、中、低剂量组裸鼠在给药8~20d内肿瘤体积明显缩小,差异均具有统计学意义(P<0.01);高、中剂量组裸鼠在给药8~20d内相对肿瘤增殖体积较模型组明显下降(P<0.01);给药20d后,高、中、低剂量组裸鼠相对肿瘤增殖率分别为8.27%,23.47%,39.42%,与模型组相比,差异均具有统计学意义(P<0.05)。结论 菌毒清颗粒可显著抑制鼻咽癌细胞CNE-2Z荷瘤裸鼠移植瘤的生长,具有明显的抗肿瘤活性。Objective To study the therapeutic effects of Junduqing extract (Junduqing granules, a kind of powder extracted from several medicinal herbs) on CNE-2Z tumor-bearing nude mice. Methods 0.2 mL CNE-2Z cell suspension was inoculated subcutaneously in right armpit of nude mice to establish transplanted tumor model. Then the nude mice were randomly divided into five groups: model control group, chlorogenic acid control group (0.01 g·kg^-1), high-dose group (8.8 g·kg^-1), medium-dose group (4.4 g·kg^-1) and low-dose group (2.2 g·kg^-1) of Junduqing extract. The rats were orally administered once per day for three weeks. The mice weight and the tumor volume were measured every other day. Three weeks later, the nude mice, the tumor tissue, spleen and thymus were weighed. The mean tumor weight, tumor growth inhibition rate, tumor volume, relative tumor volume, relative tumor proliferation rate, spleen index, thymus index and other indicators were also calculated. Results Compared with the model control group, the thymus index of nude mice was significantly increased in the high-dose, medium-dose and low-dose groups, with statistically significant differences (P<0.05). The tumors of the chlorogenic acid control group, high-dose group, medium- dose group and the low-dose group shrank in volume significantly within 8~20 days after administration, and the difference was statistically significant (P<0.01). Compared with the chlorogenic acid control group, the relative tumor proliferation volume of the high-dose group and medium-dose group decreased significantly during the 8~20 days of administration (P<0.01). After 20 days of administration, the relative tumor proliferation rates in the high, medium and low dose groups were 8.27%, 23.47% and 39.42%, respectively, showing statistically significant differences when compared with the chlorogenic acid control group (P<0.05). Conclusion Junduqing extract can obviously inhibit the growth of CNE-2Z tumor-bearing xenograft in nude mice. It has obvious anti-tumor activity.

关 键 词：菌毒清 鼻咽癌细胞CNE-2Z 裸鼠 

分 类 号：R285.5[医药卫生—中药学] R739.62[医药卫生—中医学]