大鲵源致病性弗氏柠檬酸杆菌的分离、鉴定和药物敏感性  

Isolation,identification and drug sensitivity analysis of pathogenic Citrobacter freundii from Andrias davidianus

作　　者：苏英 吴荣华[1] 申君宇 李伟平 何滔[1] 李云[1] 丁诗华[1] SU Ying;WU Rong-hua;SHEN Jun-yu;LI Wei-ping;HE Tao;LI Yun;DING Shi-hua(Southwest University,Chongqing400715,China)

机构地区：[1]西南大学动物科技学院重庆生态渔业产业技术研究院淡水鱼类资源与生殖发育教育部重点实验室

出　　处：《淡水渔业》2019年第4期56-61,共6页Freshwater Fisheries

基　　金：中央高校基本科研业务费(XDJK2016C016;SWU115061);中国博士后基金第61批面上项目资助(2017M612892);重庆市博士后科研项目特别资助(Xm20171483);重庆市基础科学与前沿技术研究专项一般项目(cstc2016jcyjA0327);重庆市农业委员会生态渔产业技术体系岗位专家项目(40810017)

摘　　要：为鉴定患病大鲵(Andrias davidianus)病原菌,从大鲵肠、胃、脾脏中分离得到菌株int1705,分离菌株经形态特征观察、生理生化特性测定、16S rRNA和aspC管家基因序列测定及系统发育学分析,鉴定为弗氏柠檬酸杆菌(Citrobacter freundii)。经人工感染,结果显示:菌株int1705能使健康大鲵发病并致死,半致死浓度(LD50)为3.16×10 6 CFU/mL,且发病症状与自然发病相似。药敏试验结果显示,菌株int1705对多粘菌素B、头孢他啶、诺氟沙星、氧氟沙星、复方新诺明等10种药物敏感。The int1705 strain was isolated from the lesion site of intestine,stomach,spleen of Andrias davidianus to identify the pathogenic agent of the diseased.The int1705 was identified as Citrobacter freundii based on morphological and biochemical identification,together with16S rRNA and aspC gene sequencing.Experimental infection results showed that int1705 exhibited virulence to Andrias davidianus with an LD50 value of3.16×10^6 CFU/mL.Symptoms were similar to those natural cases.Antimicrobial susceptibility test results showed that the int1705 was sensitive to10 kinds of antibiotics,such as polymyxin B,Ceftazidime and norfloxacin,etc.

关 键 词：大鲵(Andrias davidianus) 弗氏柠檬酸杆菌(Citrobacter freundii) 致病性 药敏试验 

分 类 号：S941.42[农业科学—水产养殖]