不同月龄牦牛睾丸中LYZL4、LYZL6的基因表达及蛋白质定位  

Gene Expression and Protein Localization of LYZL4 and LYZL6 in Testes of Different Months Old Yak

作　　者：李忠邦 阎萍[2] LI Zhongbang;YAN Ping(College of Life Science and Engineering, Northwest University for Nationalities,Lanzhou 730030,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences/Gansu Key Laboratory for Yak Breeding Engineering,Lanzhou 730050,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]中国农业科学院兰州畜牧与兽药研究所/甘肃省牦牛繁育工程重点实验室,甘肃兰州730050

出　　处：《河南农业科学》2019年第7期116-121,共6页Journal of Henan Agricultural Sciences

基　　金：中国农业科学院创新工程项目(CAAS-ASTIP-2014-LIHPS-01);现代肉牛牦牛产业技术体系项目(CARS-37)

摘　　要：为探讨人源类溶菌酶蛋白4(Human lysozyme-like protein4,LYZL4)和人源类溶菌酶蛋白6(Human lysozyme-like protein 6,LYZL6)在牦牛睾丸中的表达机制,分别选取6、18、30、72月龄健康的牦牛12头(每组3头)采集睾丸组织。应用qRT-PCR、Western-blot和免疫组化技术分别分析了LYZL4和LYZL6在不同月龄牦牛睾丸中的表达和定位。结果显示,在不同月龄牦牛睾丸中均检测到 LYZL4、LYZL 6 基因和相关蛋白质的表达,其基因表达量随着牦牛月龄增加呈现上升趋势。72、30月龄牦牛睾丸中 LYZL4、LYZL 6 基因及蛋白质的表达量极显著高于6月龄和18月龄牦牛( P < 0.01)。LYZL4蛋白在6月龄牦牛睾丸中定位于间质细胞内,在18月龄牦牛睾丸中主要定位于精原细胞和支持细胞内,在30、72月龄牦牛睾丸中定位于圆形精子细胞内。而LYZL6蛋白在6月龄牦牛的睾丸中定位于间质细胞内,在18月龄牦牛睾丸中主要定位于精原细胞内,在30、72月龄牦牛睾丸中定位于圆形精子细胞和初级精母细胞内。 LYZL4和LYZL 6 基因主要在牦牛性成熟后高丰度表达,而在性成熟前相对低丰度表达。因此,在牦牛雄性生殖系统的发育过程以及在精子发生的调控过程中LYZL4和LYZL6均发挥着重要的作用。In order to investigate the expression mechanism of human lysozyme-like protein (LYZL4) and human lysozyme-like protein (LYZL6) in yak testis,twelve yak testis tissues were collected at 6,18,30,72 months old stages (3 replicates each).The expression and localization of LYZL4 and LYZL6 in testis of different months old yak were analyzed by qRT-PCR, Western-blot and immunohistochemistry. The results showed that the expression of LYZL4, LYZL 6 genes and related proteins was detected in the testes of different months old yak, and the expression level of genes were increased with the growth of the yak age.The expression levels of LYZL4and LYZL 6 genes and proteins in the testes of 72,30 months old yak were significantly higher than those of 6 months old and18 months old yaks ( P <0.01).The immunohistochemical staining revealed that immunoreactivity of LYZL4 protein was localized at Leydig cells in 6 months old yak testis tissues,it was mainly located at spermatogonia and sertoli cells in18 months old yak testis,and it was mainly localized at spermatids in 30,72 months old yak testis tissues.The positive expression of LYZL6 protein in 6 months old yak testis tissue was localized at Leydig cells,it was mainly localized at spermatogonia in18 months old yak testis,and it was localized at spermatids and primary spermatocytes in 30,72 months old yak testis tissues.And we found that LYZL4and LYZL 6 genes reached high abundance expression after yak sexual maturity and relatively low abundance before sexual maturity.Therefore,LYZL4 and LYZL6 play an important role in the development of male reproductive system and regulation of spermatogenesis of yaks.

关 键 词：人源类溶菌酶蛋白4 人源类溶菌酶蛋白6 牦牛 睾丸 表达 定位 

分 类 号：S823.8[农业科学—畜牧学]