急性丙型肝炎患者血清IFN-λ3与HCV RNA水平之间的关系  

Relationship between serum IFN-λ3 and HCV RNA level in patients with acute hepatitis C

作　　者：张丽[1] 姜树勤 吴彩花[1] 周红霞[2] 王建华[3] ZHANG Li;JIANG Shu-qin;WU Cai-hua(Department of Infection Obstetrics,Handan Infectious disease hospital ( Handan Hebei,056002) China)

机构地区：[1]邯郸市传染病医院感染科,河北邯郸056002 [2]邯郸市传染病医院重症肝病科 [3]河北工程大学附属医院消化内科

出　　处：《中西医结合肝病杂志》2019年第3期221-224,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：河北省邯郸市科学技术研究与发展计划项目(No.1123108082-3)

摘　　要：目的:探究急性丙型肝炎(AHC)患者血清干扰素(IFN)-λ3水平与丙型肝炎病毒(HCV) RNA含量的变化及其相关性。方法:回顾性分析邯郸市传染病医院收治的159例AHC患者(急性感染组)的临床资料,另选同期体检健康者100例作为健康对照组。采用酶联免疫吸附法检测两组血清IFN-λ3水平,采用焦磷酸测序仪检测IFNL3单核苷酸多态性位点rs8099917,采用全自动荧光定量PCR仪进行HCV病毒定量检测,采用Spearman等级相关系数评估血清IFN-γ水平与HCV RNA载量的相关性;Logistic多因素回归分析影响HCV RNA含量的相关因素。结果:检测后,急性感染组患者和对照组患者血清IFN-λ3水平分别为(13.74±2.97)和(2.63±0.18)pg/ml,急性感染组患者HCV RNA载量为(5226.57±466.08)KIU/ml,差异均有统计学意义(P<0.05);AHC患者基线血清IFN-λ3水平与基线HCV RNA载量呈明显正相关(r=0.594,P=0.000)。携带rs8099917 TT基因型患者和TG/GG基因型患者基线血清IFN-λ3水平分别为(13.98±3.01)和(12.34±2.94)pg/ml,HCV RNA载量分别为(5197.83±464.26)和(5495.06±453.62)KIU/mL,差异均有统计学意义(P<0.05);Logistic多因素回归分析发现IFN-λ3是HCV RNA含量的危险因素。结论:AHC患者基线血清IFN-λ3水平的升高与血清HCV RNA载量呈正相关,IFNL3基因多态性能够影响IFN-λ3水平、促进HCV RNA载量升高进而参与AHC疾病发生、进展。Objective:To investigate the changes and correlation of serum interferon(IFN)-λ3 levels and chronic hepatitis C virus(HCV)RNA content in patients with acute hepatitis C(AHC). Methods:The clinical data of 159 patients with AHC(acute infection group) admitted to our hospital were retrospectively analyzed. Another 100 cases of physical examination at the same time as a healthy control group. Serum IFN-λ3 level was detected by enzyme-linked immunosorbent assay. Pyrosequencing was used to detect the single nucleotide polymorphism of IFNL3(rs8099917). Quantitative detection of HCV virus by using automatic fluorescence quantitative PCR instrument. Correlation between serum IFN-γ levels and HCV RNA load was assessed by Spearman rank correlation coefficient;Logistic multivariate regression analysis was used to analyze factors affecting HCV RNA content. Results:After detection,the levels of serum IFN-λ3 in the acute infection group and the control group were respectively(13.74±2.97)(2.63±0.18) pg/mL, The HCV RNA load in the acute infection group was(5226.57±466.08)KIU/ml,and the difference was statistically significant(P<0.05). Baseline serum IFN-λ3 level was positively correlated with baseline HCV RNA load in CHC patients(r=0.594,P=0.000).Patients with rs8099917 TT genotype and TG/GG genotype baseline serum IFN-λ3 levels were respectively(13.98±3.01) and(12.34±2.94)pg/mL,the load of HCV RNA were(5197.83±464.26) and(5495.06±453.62)KIU/mL,the difference was statistically significant(P<0.05).Logistic multivariate regression analysis found that IFN-λ3 is a risk factor for HCV RNA content.Conclusion:The elevated serum IFN-λ3 level in AHC patients is positively correlated with the serum HCV RNA level,IFNL3 gene polymorphism can participate in AHC disease progression and progression by affecting IFN-λ3 levels and promoting elevated HCV RNA load.

关 键 词：肝炎 丙型 急性 丙型肝炎病毒 干扰素-λ3 HCV RNA载量 

分 类 号：R512.63[医药卫生—内科学]