壳聚糖/羟基磷灰石表面修饰聚己内酯多孔骨支架的制备及性能  

Preparation and properties of polycaprolactone porous bone scaffold modified with chitosan/hydroxyapatite on the surface

作　　者：张平生[1,2] 辛勇[1] 曹传亮[1] 艾凡荣[1] ZHANG Ping-sheng;XIN Yong;CAO Chuan-liang;AI Fan-rong(School of Mechanical and Electrical Engineering,Nanchang University,Nanchang 330031,China;School of Aeronautical Manufacturing Engineering,Nanchang Hangkong University,Nanchang 330063,China)

机构地区：[1]南昌大学机电工程学院,南昌330031 [2]南昌航空大学航空制造工程学院,南昌330063

出　　处：《材料工程》2019年第7期64-70,共7页Journal of Materials Engineering

基　　金：国家自然科学基金资助项目(51365038);江西省教育厅科学技术研究资助项目(GJJ150153)

摘　　要：采用选择性激光烧结技术构建多孔聚己内酯(PCL)骨支架,用原位合成的方法制得壳聚糖/羟基磷灰石(CS/HA)悬浮液,并采用真空浸泡、低速离心和冷冻凝胶的方法使CS/HA黏附在PCL支架的表面,以改善骨支架的生物相容性和细胞增殖活性。通过X射线衍射(XRD)和扫描电子显微镜(SEM)观测复合支架的物相和形貌,测量支架的压缩强度和杨氏模量,测量支架表面的水接触角,并通过体外细胞实验研究复合支架的生物学性能。实验结果表明,原位合成的方法制得了羟基磷灰石(HA);CS/HA凝胶与PCL骨支架表面黏附良好;CS/HA改善了PCL支架表面的亲水性,提升了骨支架的生物相容性和细胞增殖活性。Porous polycaprolactone (PCL) bone scaffolds were prepared by selective laser sintering and chitosan/hydroxyapatite(CS/HA) suspension was prepared by in situ synthesis.The CS/HA was attached to the surface of the PCL scaffold by vacuum immersion,low speed centrifugation and freezing gel,to improve the biocompatibility and cell proliferation activity of the PCL scaffolds.The phase and porous structure of the composite scaffolds were observed by X ray diffraction (XRD) and scanning electron microscopy (SEM).The compressive strength and Young's modulus of the scaffolds were measured,the water contact angles on the surface of the scaffolds were measured and the biological properties of the composite scaffolds were studied by in vitro cell experiments.The experimental results show that hydroxyapatite (HA) is prepared by in situ synthesis,and CS/HA gel adhered well to the surface of PCL bone scaffold.The hydrophilicity of the surface of the PCL scaffold is improved by CS/HA,and the biocompatibility and cell proliferation activity of them are greatly enhanced.

关 键 词：聚己内酯-壳聚糖/羟基磷灰石(PCL-CS/HA) 骨支架 表面修饰 选择性激光烧结(SLS) 原位合成 冷冻凝胶 

分 类 号：TB321[一般工业技术—材料科学与工程]