一个大豆矮秆性状基因的分子标记定位  

Locating of a soybean dwarf gene by molecular markers

作　　者：袁鹰 王程 韩俊 张鑫 仝潇 冯凡育 谢皓 YUAN Ying;WANG Cheng;HAN Jun;ZHANG Xin;TONG Xiao;FENG Fanyu;XIE Hao(College of Plant Science and Technology/Beijing Key Laboratory of New Technology in Agricultural Application,Beijing University of Agriculture, Beijing 102206, China)

机构地区：[1]北京农学院植物科学技术学院/农业应用新技术北京市重点实验室

出　　处：《北京农学院学报》2019年第3期25-29,共5页Journal of Beijing University of Agriculture

基　　金：国家自然科学基金项目(31371648)

摘　　要：【目的】对大豆品种北农103中的矮秆性状进行遗传分析和初步基因定位,为该基因的精细定位和育种利用奠定基础。【方法】以高秆大豆品种海系13和矮秆品种北农103为研究材料,通过对(海系13×北农103)F4的剩余杂合体(RHL)F2分离群体的遗传分析,解析控制北农103矮秆性状基因的遗传规律,同时以(海系13×北农103)的(RHL)F2∶3为作图群体,利用BSA法结合SSR分子标记,对北农103中的矮秆基因进行分子标记和遗传定位。【结果】北农103中携带1对控制矮秆性状的隐性基因GmD1;经分子标记连锁分析,该基因与9个SSR标记连锁,分别是Satt527、Satt166、Sat_340、Satt481、Sat_150、Sat_245、Satt076、Satt229和Satt664。【结论】根据大豆遗传图谱推断,该基因位于大豆L连锁群(19染色体)上,在satt527和satt166标记之间,遗传距离分别是3.7cM和4.2cM,推测为一个新的控制大豆矮秆的基因GmD1,这些发现和分子标记定位对于该基因的精细定位将起到重要作用。【Objective】 The dwarf soybean variety BeiNong 103 was researched by genetic analysis and preliminary gene localization for fine mapping and breeding utilization.【Methods】To genetic analysis and gene mapping , a F 2:4 segregating population derived from a cross of high-stalk cultivar HaiXi 13 with a dwarf-stalk accession BeiNong 103. And the BSA methods was used as SSR primers screening and gene localization.【Results】 Genetic analysis showed that the dwarf trait of BeiNong 103 was controlled by a recessive gene GmD1 . Satt527, Satt166, Sat_340, Satt481, Sat_150, Sat_245, Satt076, Satt229 and Satt664, 9 SSR markers were found linkaged with the GmD1 gene respectively.【Conclusion】 According to the gene location, the GmD1 gene was located on the soybean L-linked group (chromosome 19), and the genetic distance were 3.7 cM 和4.2 cM between the satt527 and satt166. The speculate that GmD1 was a new dwarf gene of soybean. The results provide important information for the fine mapping of genes.

关 键 词：大豆 遗传分析 基因定位 遗传图谱 SSR标记 

分 类 号：S565.1[农业科学—作物学]