植物乳杆菌素BM-1对大肠杆菌抑菌活性的研究  

Bacteriostatic activity of plantaricin BM-1 against Escherichia coli

作　　者：张含薇 谢远红 金君华 张红星 ZHANG Hanwei;XIE Yuanhong;JIN Junhua;ZHANG Hongxing(College of Food Science and Engineering, Beijing University of Agriculture/Beijing Laboratoryof Food Quality and Safety/Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residuesin Agricultural Products, Beijing 102206, China)

机构地区：[1]北京农学院食品科学与工程学院/食品质量与安全北京实验室/农产品有害微生物及农残安全检测与控制北京市重点实验室

出　　处：《北京农学院学报》2019年第3期93-98,共6页Journal of Beijing University of Agriculture

基　　金：北京市教委科研计划项目(KM201810020016)

摘　　要：【目的】探究植物乳杆菌素BM-1对大肠杆菌的抑菌活性。【方法】通过管碟法检测植物乳杆菌素BM-1对12株大肠杆菌的抑菌活性,选取不同敏感型大肠杆菌5株,对大肠杆菌生长曲线、活菌数、生物膜的形成进行测定,并采用实时荧光定量PCR方法测定大肠杆菌中细菌素受体基因的表达。【结果】生长曲线、活菌数、生物膜的形成试验结果显示,大肠杆菌CGMCC1.8723对植物乳杆菌素BM-1敏感性最强;实时荧光定量PCR显示植物乳杆菌素BM-1显著影响大肠杆菌甘露糖磷酸转移酶系统编码基因的表达。【结论】植物乳杆菌素BM-1对大肠杆菌表现出不同水平的抑菌活性。【Objective】 In this study the bacteriostatic activity ofplantaricin BM-1 against Escherichia coli was investigated.【Method】 The bacteriostatic activity of plantaricin BM-1 on Escherichia coli was detected by Oxford Cup bacteriostatic method. Five Escherichia coli strains were selected from different sensitive to plantaricin BM-1, and their growth curves, viable counts, biofilm formation and real-time PCR were determined.【Result】 Growth curve, viable count and biofilm formation showed that CGMCC 1.8723 was the most sensitive strain to plantaricin BM-1. Real-time PCR showed that plantaricin BM-1 stimulated the receptor gene expression.【Conclusion】 Plantaricin BM-1 exhibited different levels of antibacterial activity against Escherichia coli .

关 键 词：植物乳杆菌素BM-1 大肠杆菌 甘露糖磷酸转移酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]