二至丸抗大鼠肾细胞衰老的GC-MS代谢组学研究  

Metabolomics Study of Erzhiwan Against Normal Rat Kidney Cell Senescence Based on GC-MS

作　　者：徐佳[1,2,3] 刘其南 翟园园[3] 冯丽 谢彤[1,2] 姚卫峰[2,3] 张丽 XU Jia;LIU Qi-nan;ZHAI Yuan-yuan;FENG Li;XIE Tong;YAO Wei-feng;ZHANG Li(Jiangsu Key Laboratory of Pediatric Respiratory Disease,Institute of Pediatrics,Nanjing University of Chinese Medicine,Nanjing,210023,China;Medical Metabolomics Center,Nanjing University of Chinese Medicine,Nanjing,210023,China;Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing,210023,China)

机构地区：[1]江苏省儿童呼吸疾病(中医药)重点实验室,南京中医药大学中医儿科学研究所,江苏南京210023 [2]南京中医药大学医学代谢组学中心,江苏南京210023 [3]南京中医药大学江苏省方剂高技术研究重点实验室,江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《南京中医药大学学报》2019年第4期458-464,共7页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(85173554);江苏省“六大人才高峰”高层次人才项目(YY026);江苏高校青蓝工程中青年学术带头人项目;江苏省研究生科研与实践创新计划项目(KYCX18_1585)

摘　　要：目的研究D-半乳糖诱导的衰老大鼠肾细胞(NRK)内源性代谢物的变化,寻找与衰老有关的代谢生物标志物,探讨二至丸对衰老模型的调节作用及其抗衰老的可能机制.方法采用D-半乳糖复制NRK细胞衰老模型,借助气相色谱-质谱联用(GC-MS)技术,对各组细胞样本进行测定,采用Kruskal-Wallis非参数检验及Dunn's多重比较进行单变量统计分析来筛选差异性代谢物.结果经PLS-DA模式识别分析,对照组、模型组和EZW组细胞样本能够得到很好的区分,共鉴定出31种潜在生物标志物,二至丸含药血清给药后衰老NRK细胞胞内的内源性代谢物水平发生不同程度的回调.结论二至丸可以使D-半乳糖诱导的衰老NRK细胞胞内的异常代谢有所恢复,其治疗作用可能与机体内31个代谢物及10条相关代谢通路的调节有关.OBJECTIVE To investigate the changes of endogenous metabolites in senile rat kidney cells (NRK) induced by D galactose, to find metabolic biomarkers related to aging and to explore the regulation of Erzhiwan on aging model and the anti aging effect of Erzhiwan. METHODS The senescence model was constructed by D galactose (D-gal). The gas chromatography mass spectrometry (GC-MS) technique was used to determine the cell samples in each group. Univariate statistical analysis was performed using the Kruskal Wallis nonparametric test and Dunn's multiple comparison. RESULTS PLS-DA pattern recognition analysis showed that the cell samples of the control group, the model group and the EZW group could be well distinguished. A total of 31 potential biomarkers were identified, and the endogenous metabolite levels of the senescent NRK cells were varying degrees of callbacks after the administration of the drug containing serum. CONCLUSION Erzhiwan could restore the abnormal metabolism of D- galactose induced senescent NRK cells, and its therapeutic effect may be related to the regulation of 31 metabolites and 10 related metabolic pathways.

关 键 词：衰老 二至丸 细胞代谢组学 GC-MS 大鼠肾细胞 含药血清 

分 类 号：R285.5[医药卫生—中药学]