RETN、IFN-γ、TNF-α及TGF-β在痛风性关节炎患者外周血和滑膜液中的表达及意义  

Expression and significance of RETN, IFN-γ, TNF-α and TGF-β in peripheral blood and synovial fluid of gouty arthritis patients

作　　者：赖爱云[1] 徐健[1] 陶丽[1] LAI Aiyun;XU Jian;TAO Li(Department of Rheumatology, The First Affiliated Hospital of Kunming Medical University,Kunming 650032,China)

机构地区：[1]昆明医科大学第一附属医院风湿免疫科

出　　处：《西部医学》2019年第7期1082-1087,共6页Medical Journal of West China

基　　金：云南省卫生科技计划项目(2016NS051)

摘　　要：目的探讨痛风性关节炎患者外周血及滑膜液中尿酸(UA)、抵抗素(RETN)、γ-干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)及转化生长因子-β(TGF-β)的表达水平及其临床意义。方法选择本院2016年5月~2017年7月收治的112例痛风性关节炎患者(40例急性期、35例慢性期和37例间歇期患者)为痛风性关节炎组,另选择35例骨性关节炎患者(骨性关节炎组)和35例身体健康者为对照组。用酶联免疫(ELISA)方法检测各组外周血及滑膜液中UA、RETN、IFN-γ、TNF-α及TGF-β水平,并分析其与临床病理特征的关系。结果与骨性关节炎组及健康对照组比较,痛风性关节炎组患者外周血及滑膜液中UA、RETN、IFN-γ、TNF-α及TGF-β水平显著升高(P<0.05)。与慢性期比较,急性期痛风患者外周血及滑膜液中RETN、IFN-γ、TNF-α及TGF-β水平均显著升高(P<0.05);与间歇期比较,慢性期患者外周血及滑膜液中UA、TNF-α、TGF-β水平亦显著升高(P<0.05)。与病程<5年组比较,病程≥5年组患者外周血中UA、IFN-γ、TNF-α及TGF-β水平均显著升高(P<0.05);滑膜液中RETN、IFN-γ、TNF-α及TGF-β水平亦显著升高(P<0.05)。与受累关节数<5个组比较,受累关节数≥5个组患者外周血中UA、RETN及TNF-α水平均显著升高(P<0.05);滑膜液中RETN、IFN-γ、TNF-α及TGF-β水平亦显著升高(P<0.05)。与发作频率<3次/12个月组比较,发作频率≥3次/12个月组患者外周血中UA、RETN及TNF-α水平均显著升高(P<0.05);滑膜液中UA、RETN、TNF-α及TGF-β水平亦显著升高(P<0.05)。痛风患者血清及滑膜液中UA水平与RETN、IFN-γ、TNF-α及TGF-β呈正相关(P<0.05)。结论 RETN、IFN-γ、TNF-α及TGF-β在痛风性关节炎患者外周血及滑膜液中均呈高表达,且病情与发展程度有关,提示其在痛风性关节炎的炎症反应过程中可能发挥了重要作用。Objective To detect the expression levels of resistin(RETN), interferon-γ(IFN-γ), tumor necrosis factor-α(TNF-α) and transforming growth factor-β(TGF-β) in peripheral blood and synovial fluid of patients with gouty arthritis, and explore their clinical significance. Methods 112 patients with gouty arthritis admitted in our hospital from May 2016 to July 2017 were selected, including 40 cases of acute phase, 35 cases of chronic phase and 37 cases of intermittent period, another 35 patients with osteoarthritis and 35 healthy persons were selected as the control group. The levels of RETN, IFN-γ, TNF-α and TGF-β in peripheral blood and synovial fluid were detected by enzyme linked immunosorbent assay(ELISA), their relationships with the clinicopathological features were analyzed. Results Compared with the osteoarthritis group or the healthy control group, the levels of UA, RETN, IFN-γ, TNF-α and TGF-β in the peripheral blood and synovial fluid of gouty arthritis group were significantly increased(P<0.05). Compared with the chronic phase, the levels of RETN, IFN-γ, TNF-α and TGF-β in the peripheral blood and synovial fluid of the patients in the acute phase were significantly increased(P<0.05). Compared with the intermittent period, the levels of UA, TNF-α and TGF-β in the peripheral blood and synovial fluid of the patients in the chronic period were significantly increased(P<0.05). Compared with the course<5 years group, the levels of UA, IFN-γ, TNF-α and TGF-β in the peripheral blood of patients in course ≥5 years group were significantly increased(P<0.05), the levels of RETN, IFN-γ, TNF-α and TGF-β in synovial fluid were increased significantly(P<0.05). Compared with the number of affected joints<5 group, the levels of UA, RETN and TNF-α in the peripheral blood of patients in the number of affected joints ≥5 group were significantly increased(P<0.05), the levels of RETN, IFN-γ, TNF-α and TGF-β in synovial fluid were increased significantly(P<0.05). Compared with the frequency of seizu

关 键 词：痛风性关节炎 抵抗素 Γ-干扰素 肿瘤坏死因子-α 转化生长因子-Β 

分 类 号：R593.2[医药卫生—内科学]