草莓抗枯萎病分子标记初定位  

The preliminary screening on molecular marker of Fusarium oxysporum resistance of strawberry

作　　者：史芳芳 SHI Fang-fang(Institute of Agricultural Sciences of the 12th Division,Xinjiang Production and Construction Corps,Urumqi 830088,China)

机构地区：[1]新疆生产建设兵团第十二师农业科学研究所

出　　处：《湖北农业科学》2019年第13期72-75,共4页Hubei Agricultural Sciences

基　　金：十二师农业科技攻关项目“草莓优质抗病早熟新品种(系)的选育”(SR2016018);兵团重点领域创新团队计划项目“兵团草莓脱毒与繁育技术体系研究创新团队”(2016BD006)

摘　　要：为加快草莓(Fragaria×ananassaDuch.)枯萎病抗病分子育种进程,以草莓优系后代为试材进行抗病试验分析,对父母本及不同优系后代进行抗病基因的筛选,并结合SSR分子标记技术,最终筛选出抗病品系。结果表明,抗病试验中,优系13-53-100和13-52-407具有很强的抗枯萎病特性,优系13-65-265抗枯萎病特性最差;抗病基因筛选出亲本中紫金四季和甜查理含有抗病性基因,优系后代中13-53-100和13-52-407含有抗病基因,可能遗传了甜查理的抗病性。In order to speed up the molecular breeding process of strawberry resistance(Fragaria×ananassa Duch.), the excellent hybrid offspring of strawberry were used as the test materials. At first,the resistance test was carried out, and the resistant genes of parents and different hybrid offsprings were screened, combined with SSR molecular marker technology. Finally, the resistant strains were screened out. The results showed that excellent offspring 13-53-100 and 13-52-407 had strong resistance to F. oxysporum, and excellent offspring 13-65-265 had the worst resistance to F. oxysporum. The screening result of resistance gene showed that, the parent Zijin Sijin and sweet Charlie had the resistance gene, the offspring 13-53-100 and 13-52-407 had a consistent band of parent sweet Charlie, which may have inherited the resistance of sweet Charlie.

关 键 词：草莓(Fragaria×ananassaDuch.) 枯萎病 抗性基因 SSR分子标记 

分 类 号：S668.4[农业科学—果树学] S436.68[农业科学—园艺学]