HPLC法测定芳香化湿口服液中的橙皮苷含量  

Determination of hesperidin in aromatic Huashi oral liquid by HPLC

作　　者：梁晓[1] 赵小媛 李岩涛 陈睿[1] LIANG Xiao;ZHAO Xiao-yuan;LI Yan-tao;CHEN Rui(Baoji Traditional Chinese Medicine Hospital,Baoji 721000,China)

机构地区：[1]宝鸡市中医医院

出　　处：《临床医学研究与实践》2019年第20期131-133,共3页Clinical Research and Practice

摘　　要：目的建立高效液相色谱法(HPLC)测定医疗机构制剂芳香化湿口服液的含量控制标准,以控制该制剂的质量。方法采用DiamonsilTMC18色谱柱;乙腈-0.12%磷酸溶液(20∶80)为流动相;流速为1.0mL/min;检测波长为284nm。结果此色谱条件下,可以完全分离橙皮苷。橙皮苷回归方程为Y=1751.1X+116488,相关系数r=0.9999。橙皮苷进样量在122~976ng范围内线性关系良好(r=0.9999),平均加样回收率为99.99%,RSD=0.88%。结论HPLC法操作简便、专属性强、重现性好,可有效控制芳香化湿口服液的质量。Objective To establish the content control standard of aromatic Huashi oral liquid in medical institutions by high performance liquid chromatography(HPLC),in order to control the quality of the preparation.Methods Diamonsil for the determination TMC18 column was used,acetonitrile-0.12%phosphoric acid solution(20∶80)was used as mobile phase,the flow rate was 1.0 mL/min,and the detection wavelength was 284 nm.Results Under this chromatographic condition,hesperidin could be completely separated.The regression equation of hesperidin was Y=1751.1X+116488,and the correlation coefficient was 0.9999.The linear relationship between the amount of hesperidin in the range of 122-976 ng was good(r=0.9999).The average recovery of hesperidin was 99.99%and RSD=0.88%.Conclusion HPLC is simple,specific and reproducible,which can effectively control the quality of aromatic Huashi oral liquid.

关 键 词：橙皮苷 高效液相色谱 含量测定 

分 类 号：R286.0[医药卫生—中药学]