文山牛Y染色体遗传多样性与父系起源研究  

Genetic Diversity of Y Chromosome and Paternal Origin of Y-SNPs and Y-STRs in Wenshan Cattle

作　　者：亐开兴[1] 王凯悦 张继才[1] 黄必志[1] 陈宁博[2] 冯光杰 李顺东 陈宏[2] 雷初朝[2] QU Kai-xing;WANG Kai-yue;ZHANG Ji-cai;HUANG Bi-zhi;CHEN Ning-bo;FENG Guang-jie;LI Shun-dong;CHEN Hong;LEI Chu-zhao(Yunnan Academy of Grassland and Animal Science,Kunming 650212;College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100;Yunnan Guduo Agriculture and Animal Husbandry Co.Ltd.,Guangnan,Yunnan 663300)

机构地区：[1]云南省草地动物科学研究院,昆明650212 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]云南谷多农牧业有限公司,云南广南663300

出　　处：《中国牛业科学》2019年第3期1-4,共4页China Cattle Science

基　　金：云南省后备人才培养计划项目(2018HB045);重点研发(农业领域)项目(2018BB028);国家肉牛牦牛产业技术体系资助项目(CARS-37)

摘　　要：[目的]探究云南文山牛群体Y染色体遗传结构与血统来源,以期为该黄牛品种的资源保护与利用提供科学依据。[方法]研究采用PCR扩增和生物信息学方法,对55头文山牛Y-SNPs(UTY19和ZFY10)和Y-STRs(INRA189和BM861)遗传多样性进行系统研究。[结果]55头文山牛均属于瘤牛Y3单倍型组,结合Y-SNPs和Y-STRs分型结果,发现文山牛中存在Y3-88-156和Y3-90-156两种Y染色体单倍型,Y染色体单倍型多样度为0.1684±0.0636。[结论]云南文山牛只有瘤牛父系起源,其遗传血统稳定,纯合度高。[Objective]The genetic structure and paternal origins of Y chromosome in Wenshan cattle were analyzed to provide a scientific basis for the resource protection and utilization of this breed.[Method]PCR amplification and bioinformatics methods were used to detect the genetic diversity of Y-SNPs(UTY19 and ZFY10)and Y-STRs(INRA189 and BM861)in 55 Wenshan cattles.[Results]All the cattle belong to zebu Y3 haplogroup,and two Y chromosome haplotypes(Y3-88-156 and Y3-90-156)were identified by combining Y-SNPs and Y-STRs genotyping data.The Y chromosome haplotype diversity in Wenshan cattle was 0.1684±0.0636.[Conclusion]Wenshan cattle only had paternal origin of zebu with stable genetic lineage and high homozygosity.

关 键 词：文山牛 Y-SNPS Y-STRS 遗传多样性 父系起源 

分 类 号：S823.81[农业科学—畜牧学] Q347[农业科学—畜牧兽医]