桦褐孔菌乙酸乙酯提取物柱层析分离产物检测  

Detection of Product Isolated from Crude Ethyl Acetate Extract of Inonotus obliqu

作　　者：李咏欣 朱长俊[1] 张运洋[2] 孙俪容 茆轩宇 李子龙 朱红薇[1] LI Yong-xin;ZHU Chang-jun;ZHANG Yun-yang;SUN Li-rong;MAO Xuan-yu;LI Zi-long;ZHU Hong-wei(College of Biological and Chemical Engineering in Jiaxing University, Zhejiang Jiaxing 314001;Wuhe County Hospital of Traditional Chinese Medicine, Anhui Wuhe 233300, China)

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [2]安徽省五河县中医院,安徽五河233300

出　　处：《广州化工》2019年第13期117-119,122,共4页GuangZhou Chemical Industry

基　　金：2018年嘉兴学院校级重点SRTB类项目(SRT2018B070);2018年嘉兴学院国家级大学生创新创业训练计划项目(201810354021)

摘　　要：利用超声破壁处理桦褐孔菌子实体,粉末于90%乙醇浸泡168h后过滤。用石油醚、乙酸乙酯、正丁醇依次萃取上层溶液得到石油醚、乙酸乙酯、正丁醇粗提物。经检测乙酸乙酯粗提物有较好的抑菌和抗氧化效果。将乙酸乙酯粗提物在硅胶柱中进行梯度洗脱得到4种组分,确定其中1,2,3号产物有抑菌作用,4种均有抗氧化作用,2号分离产物抗氧化效果最好。核磁共振检测显示均为萜类化合物。The fruiting bodies of Inonotus obliquus were treated by ultrasonic wave breaking, and the powder was soaked in 90% ethanol for 168 h and then filtered. The upper layer solution was sequentially extracted with petroleum ether, ethyl acetate and n-butanol to obtain petroleum ether, ethyl acetate and crude n-butanol. The crude ethyl acetate extract had good antibacterial and antioxidant effects. The crude ethyl acetate extract was subjected to gradient elution on a silica gel column to obtain four components, and it was confirmed that the products of No.1, 2 and 3 had antibacterial action, four of which had antioxidant effect, and the antioxidant effect of No.2 isolated product was the best. NMR tests showed that they were all terpenoids.

关 键 词：桦褐孔菌 硅胶柱色谱法 产物检测 

分 类 号：R93[医药卫生—生药学]