硅烷功能化碳点荧光探针检测槲皮素  

Detection of Quercetin Using a Fluorescence Probe Based on Organosilane-functionalized Carbon Dots

作　　者：杜青青 张平平 谈琼 陈月丹 DU Qing-qing;ZHANG Ping-ping;TAN Qiong;CHEN Yue-dan(School of Pharmacy,Qilu Medical University,Zibo255300,China;Key Laboratory of Biomedical Engineering&Technology in Universities of Shandong,Zibo255300,China)

机构地区：[1]齐鲁医药学院药学院,山东淄博255300 [2]山东省高校生物医学工程技术重点实验室,山东淄博255300

出　　处：《分析测试学报》2019年第7期835-839,共5页Journal of Instrumental Analysis

基　　金：山东省自然科学基金项目(ZR2018PC033);山东省高等学校科研计划项目(J16LA51);山东省高等学校青年教师成长计划项目

摘　　要：以柠檬酸为碳源,硅烷偶联剂为表面包覆剂,一步水热法合成了高效发光硅烷功能化碳点。所得碳点在360nm激发后在450nm处有强荧光发射峰,荧光量子产率最高可达69.2%。基于槲皮素对该碳点荧光的猝灭作用,建立了一种以硅烷碳点为荧光探针的简便、灵敏检测槲皮素的分析方法。考察了作用时间、pH值、碳点用量对槲皮素检测的影响,并探讨了荧光猝灭机制。在优化实验条件下,该方法对槲皮素的检测线性范围为1.0~40.0μmol/L,相关系数(r)为0.9967,检出限为3.8nmol/L。该方法应用于实际样品中槲皮素的测定,结果满意。A simple and efficient method for the detection of quercetin was established based on the fluorescence quenching effect of quercetin toward organosilane-functionalized carbon dots(SiCDs).The highly luminescent SiCDs were prepared through one-step hydrothermal method,with citric acid as carbon source and organosilane as surface coating agent.The as-synthesized SiCDs showed a strong emission at 450 nm when excited at 360 nm,and the fluorescence quantum yield was up to 69.2 %.Effects of action time,pH value and carbon dots concentration on the detection of quercetin were investigated.Furthermore,the fluorescence quenching mechanism was elucidated.Under the optimal conditions,a linear fluorescence signal response to quercetin concentration was obtained in the range of 1.0-40.0 μmol/L with a correlation coefficient( r ) of 0.996 7,and the detection limit was 3.8 nmol/L.The method was applied in the detection of quercetin in practical samples with satisfactory results.

关 键 词：硅烷功能化碳点(SiCDs) 荧光猝灭 纳米探针 槲皮素 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]