γ-氨基丁酸键合硅胶毛细管色谱柱填料的绿色制备及其分析性能研究  

Green Preparation of a γ-Aminobutyric Acid Bonded Silica Packing Material for Capillary Column and Its Performance Evaluation

作　　者：赵双丽 罗菲菲 祝璐琪 王清江 何品刚 ZHAO Shuang-li;LUO Fei-fei;ZHU Lu-qi;WANG Qing-jiang;HE Pin-gang(School of Chemistry and Molecular Engineering,East China Normal University,Shanghai200241,China)

机构地区：[1]华东师范大学化学与分子工程学院

出　　处：《分析测试学报》2019年第7期865-869,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21575042)

摘　　要：该文在水相溶剂中绿色合成了γ-氨基丁酸键合硅胶(Sil-GABA)色谱固定相,采用红外光谱、元素分析等对其进行了表征,并将制备的Sil-GABA材料作为毛细管色谱柱的填料研究了其色谱分离模式和分析性能。结果显示,在以乙酸铵水溶液为流动相的条件下,该填料可有效分离硝基氯苯3种位置异构体和7种磺胺,表明Sil-GABA固定相具有富水作用色谱的特性;在高浓度有机流动相条件下可有效分离4种苯甲酸类化合物,表明Sil-GABA具有亲水作用色谱的特性;此外,该Sil-GABA固定相在反相模式下对硝基苯胺异构体也有一定的分离能力。在优化条件下,7种磺胺保留时间的相对标准偏差小于3.2%,表明制得的色谱柱具有良好的重复性。该Sil-GABA固定相具有多种保留机理,在毛细管液相色谱分离领域具有潜在的应用价值。A γ-aminobutyric acid bonded silica(Sil-GABA) stationary phase was prepared in a green medium with only water as solvent,which was characterized by Fourier transform infrared spectroscopy and elemental analysis.The separation mode and analytical performance of the Sil-GABA stationary phase were evaluated through a series of experiments when it was used as packing material for capillary column.Results showed that three nitrochlorobenzene positional isomers and seven sulfonamides were separated effectively on the Sil-GABA stationary phase with ammonium acetate as mobile phase in an aqueous liquid chromatography mode.Four benzoic acid compounds were separated with high concentrations of organic solvents as mobile phases in a hydrophilic chromatography mode.Additionally,three nitroaniline positional isomers were also separated in a reversed phase chromatography mode.Under the optimal conditions,the relative standard deviations(RSD) of the retention times for the sulfonamides were within 3.2 %,indicating that the Sil-GABA column has a good reproducibility.The prepared Sil-GABA stationary phase could provide potential applications in capillary liquid chromatography with multiple retention mechanisms.

关 键 词：γ-氨基丁酸键合硅胶 绿色合成 毛细管液相色谱 

分 类 号：O657.7[理学—分析化学]