融合肽TAT-Helicokinin I对舞毒蛾生物活性研究  

Bioactivity of TAT-Helicokinin I fusion peptide to larvae of Lymantria dispar

作　　者：李永丽[1] 闫作炳 周洲[1] 张永安[2] 曲良建[2] LI Yongli(College of Forestry,Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091

出　　处：《中国森林病虫》2019年第4期18-21,共4页Forest Pest and Disease

基　　金：中国林业科学研究院森林生态环境与保护研究所基本科研业务费专项资金(CAFRIFEEP201501);国家自然科学基金(31600519,31870638)

摘　　要：以美洲棉铃虫Helicoverpa zea利尿激肽Helicokinin I和穿膜肽TAT为基础合成融合肽TAT-Helicokinin I,并测试3种肽对舞毒蛾Lymantria dispar幼虫的注射活性;探究融合肽TAT-Helicokinin I和利尿激肽Helicokinin I经取食途径进入虫体后对舞毒蛾幼虫生长的影响。结果表明:TAT-Helicokinin I和Helicokinin I对舞毒蛾幼虫均表现出注射毒性,且TAT-Helicokinin I比Helicokinin I表现出更高的生物活性;60,600 pmol/头的剂量对舞毒蛾幼虫均表现出显著致死性,6 pmol/头的剂量对舞毒蛾幼虫生长无影响;Helicokinin I饲喂舞毒蛾幼虫未显现出毒性,而饲喂TAT-Helicokinin I对试虫生长表现出显著抑制作用。该研究证实TAT与Helicokinin I融合后能够降低昆虫消化道酶对利尿激肽Helicokinin I的降解。The peptide of Helicokinin I from Helicoverpa zea was synthesized as a fusion peptide with a transactivator of transcription(TAT)protein.Bioactivity of fusion peptide of TAT-Helicokinin I to larvae of gypsy moth was evaluated by injection and feeding.Results indicated that Lymantria dispar larvae injected TAT-Helicokinin I or Helicokinin I exhibited a significantly reduced survival rate.TAT-Helicokinin I showed greater bioactivity than Helicokinin I.The dose of 60 and 600 pmol showed significant lethality to test larvae.The dose of 6 pmol showed no lethality to test larvae.TAT-Helicokinin I was spread on the artificial diet,and its growth-inhibiting effect was determined.However,single peptide of Helicokinin I did not show toxicity to larvae of gypsy moth by feeding.The fusion peptide TAT-Helicokinin I showed toxicity to gypsy moth larvae in injection and feeding test.The results indicated that the fusion for TAT and Helicokinin can reduce the degradation by insect digestive tract efficiently.

关 键 词：舞毒蛾 TAT蛋白转导域 利尿激肽 

分 类 号：Q965.9[生物学—昆虫学]