不同选择性的C18检测全鹿丸中补骨脂素和异补骨脂素含量  

Determination of Psoralen and Isopsoralen in Alrodeer Pill by C18 with Different Selectivity

作　　者：吴小梅 刘征宙 郭德勇 卢晓伟 陈再洁 任兴发 屠炳芳 WU Xiao-mei;LIU Zheng-zhou;GUO De-yong;LU Xiao-wei;CHEN Zai-jie;REN Xing-fa;TU Bing-fang(Welch Material, Shanghai 201203, China;Sinopharm Chemical Reagent Co.,Ltd. ,Shanghai 200002,China)

机构地区：[1]月旭科技(上海)股份有限公司,上海201203 [2]国药集团化学试剂有限公司,上海200002

出　　处：《化学试剂》2019年第7期725-728,共4页Chemical Reagents

摘　　要：研究了用不同键合工艺的C18柱在药典要求的流动相条件下对全鹿丸中目标物进行分离的不同效果,通过使用不同选择性的色谱柱对全鹿丸中的补骨脂素和异补骨脂素进行含量测定,建立了高效液相色谱法测定中药成分全鹿丸中补骨脂素和异补骨脂素的方法。方法简便、灵敏、准确、重现性好、分析时间短,满足药企对全鹿丸的质量控制要求。The effect of C18 column with different bonding processes on the separation of target substances in alrodeer pill with the mobile phase required by Chinese pharmacopoeia was investigated.The method for the determination of psoralen and isopsoralen in alrodeer pill by high performance liquid chromatography was established by using different selective columns. And the results showed the method was simple,sensitive,accurate,reproducible,and had short analysis time. Therefore,it can meet the quality control requirement of pharmaceutical enterprises for alrodeer pill.

关 键 词：高效液相色谱法 C18柱 全鹿丸 补骨脂素 异补骨脂素 中药成分测定 质量标准 

分 类 号：R917[医药卫生—药物分析学]