黄藤素对急性肺损伤小鼠肺组织NF-B蛋白表达和激活的影响  

Effect of Fibrauretin on the protein expression and activation of NF-κB in lung tissues of mice with acute lung injury

作　　者：李莉[1] 王应霞[2] 杨桂梅 尹健彬 张旋 LI Li;WANG Yingxia;YANG Guimei;YIN Jianbin;ZHANG Xuan(Biotherapy Center, the 3rd Affiliated Hospital of Kunming Medical University Tumor Hospital of Yunnan Province, Yunnan Province, Kunming 650106, China;Department of Pathology, the First Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650032, China;School of Pharmaceutical Sciences & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Yunnan Province, Kunming 650500, China)

机构地区：[1]昆明医科大学第三附属医院云南省肿瘤医院生物治疗中心,云南昆明650106 [2]昆明医科大学第一附属医院病理科,云南昆明650032 [3]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500

出　　处：《中国医药导报》2019年第19期7-10,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81460565);云南省中青年学术和技术带头人培养项目(2015HB042);云南省科技厅-昆明医科大学应用基础研究联合专项(2013FB111)

摘　　要：目的研究黄藤素对急性肺损伤(ALI)小鼠肺组织核因子-κB(NF-κB)表达和激活的影响。方法雄性SPF昆明小鼠60只按照抽签法随机分为6组(每组10只):ALI组[气管滴入5 mg/kg脂多糖(LPS)],正常对照组(假手术处理),阳性对照组(3.3 mg/kg氢化可的松注射液+LPS),黄藤素低、中、高剂量组(2、10 mg/kg和50 mg/kg黄藤素注射液+LPS)。阳性药物和黄藤素均在模型建立前腹腔注射,1次/d,连续3 d。采用免疫组织化学法检测NF-κB p65蛋白在肺组织中的表达与定位。采用Western blot法检测磷酸化NF-κB p65蛋白的表达情况。结果与ALI组比较,黄藤素中、高剂量组小鼠肺组织中NF-κB p65蛋白进入细胞核的阳性细胞百分率明显下降,差异有统计学意义(P <0.05)。与ALI组比较,黄藤素低、中、高剂量组小鼠肺组织磷酸化NF-κB p65蛋白表达明显下调,差异有统计学意义(P <0.05或P <0.01)。结论黄藤素对LPS诱导的小鼠急性肺损伤具有防治作用,该作用与抑制NF-κB蛋白表达和激活有关。Objective To study the effect of Fibrauretin on the protein expression and activation of nuclear factor kappa B(NF-κB) in lung tissues of mice with acute lung injury(ALI). Methods Sixty male SPF Kunming mice were randomly divided into 6 groups by drawing lots(10 mice in each group): ALI group [(intratracheal instillation of 5 mg/kg lipopolysaccharide(LPS)], normal control group(sham operation), positive control group(3.3 mg/kg Hydrocortisone Injection + LPS),low, medium and high doses of Fibrauretin groups(2, 10 mg/kg and 50 mg/kg Fibrauretin Injection + LPS). The positive drug and Fibrauretin were given by intraperitoneal injection once a day for 3 consecutive days before establishment of ALI model. Immunohistochemistry was used to detect the expression and localization of NF-κB p65 protein in lung tissues. Western blot was used to detect the expression of phosphorylated NF-κB p65 protein in lung tissues. Results Compared with ALI group, the percentage of positive cells with the entry of NF-κB p65 protein into the nucleus in lung tissues of mice in medium and high doses of Fibrauretin groups was decreased significantly, the difference was statistically significant(P < 0.05). Compared with ALI group, the protein expression of phosphorylated NF-κB p65 in lung tissues of mice in low, medium and high doses of Fibrauretin group was down-regulated significantly, the differences were statistically significant(P < 0.05 or P < 0.01). Conclusion Fibrauretin has preventive effect on LPS-induced acute lung injury in mice, and the effect is related to the inhibition of the protein expression and activation of NF-κB.

关 键 词：黄藤素 急性肺损伤 脂多糖 核因子-ΚB 蛋白表达 

分 类 号：R563.1[医药卫生—呼吸系统]