控释神经生长因子的阵列微管促进神经再生  

Nerve scaffold with longitudinally oriented micro-channels and controlled-release of NGF for promoting peripheral nerve regeneration

作　　者：石晓伟 黄亮亮 夏冰 高楗勃 杨雨洁 罗卓荆 黄景辉 SHI Xiao-wei;HUANG Liang-liang;XIA Bing;GAO Jian-bo;YANG Yu-jie;LUO Zhuo-jing;HUANG Jing-hui(Department of Orthopaedics,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学西京医院骨科

出　　处：《中国矫形外科杂志》2019年第13期1205-1210,共6页Orthopedic Journal of China

基　　金：国家自然科学基金项目(编号:81672148;81201389);国家重点研发计划项目(编号:2016YFC1101700);国家重点基础研究项目(973计划,编号:2014CB542206);长江学者和创新发展团队计划项目(编号:IRT1053;IRT13051);全国优秀博士论文基金项目(编号:201480)

摘　　要：[目的]制备可控释神经生长因子(nerve growth factor,NGF)的阵列微管神经修复支架,并评价其促神经再生效能。[方法]采用梯度冷凝技术制备复合NGF的阵列微管神经修复支架,检测其机械特性和NGF释放规律。将新生SD大鼠的背根神经节(dorsal root ganglions,DRG)随机分为4组:无序支架组、复合NGF的无序支架组、阵列微管支架组和复合NGF的阵列微管支架组。将DRG植入各组支架中体外培养72 h,观察轴突生长情况。[结果]在机械强度方面,各组在横向压缩过程中的峰值载荷差异无统计学意义(P>0.05)。纵向牵拉测试中,神经支架组与自体神经组的应力-应变曲线类似。在NGF释放规律方面,阵列微管组和无序组的释放曲线类似,且不同时间点NGF浓度差异无统计意义(P>0.05)。在轴突生长方面,复合NGF的阵列微管支架组轴突长度为(1 025.41±175.33)μm,显著高于阵列微管支架组(857.58±233.14)μm、复合NGF无序支架组(341.73±52.27)μm、无序支架组(225.36±61.62)μm。[结论]复合NGF的阵列微管神经修复支架同时具有营养活性及引导结构,且具有良好的机械性能,在体外环境下可以引导大鼠DRG轴突定向延伸,在周围神经再生领域具有重要前景。Abstract:[Objective]To fabricate a novel nerve scaffolds with longitudinally oriented micro-channels and controlled-release of NGF,and evaluate its effect on promoting nerve regeneration.[Methods]A directional freezing-dry technique was used to fabricate a novel nerve scaffold with longitudinally oriented micro-channels and controlled release of NGF.The mechanical properties and time course of NGF release of the nerve scaffolds were evaluated.The dorsal root ganglia(DRGs)of neonate rats were randomly divided into 4 groups:the random scaffold group,the random scaffold+NGF group,the scaffold with micro channels group,and the scaffold with micro channels+NGF group.The neurite length was measured at 72h of culture in those 4 groups.[Results]In term of mechanical strength,no significant difference was observed in max load of during transverse compression testing among the groups(P=0.462),and the stress-strain curve of the scaffold group was in the range similar to that of the autologous nerve during longitudinal distraction testing.In term of the time course of NGF release,the curve of NGF release was similar between the scaffold with micro channels+NGF group and random scaffold+NGF group,without a significant difference in NGF concentration between the two groups at different time points(P=0.685).The ingrowth length of axons in the scaffold with micro channels+NGF group was of(1 025.41±175.33)μm in longitudinal orientation,which was significantly longer than that in the random group of(225.36±61.62)μm,the random scaffold+NGFgroup of(341.73±52.27)μm,and the scaffold with micro channels group of(857.58±233.14)μm(P<0.05).[Conclusion]The nerve scaffold with longitudinally oriented micro-channels and controlled-release of NGF is of neurotrophic activity and excellent mechanical properties,which is capable of guiding neurite outgrowth and has great potential in promoting peripheral nerve regeneration.

关 键 词：丝素蛋白 神经生长因子 阵列微管 神经支架 背根神经节 

分 类 号：R318[医药卫生—生物医学工程]