子痫前期孕妇胎盘组织中miR-205表达的研究  

Investigation of miR-205 expression in placenta of pregnant woman with preeclampsia

作　　者：陈文婷 李仲均[1] 陈静华[1] 曾带娣 黄素然[1] 萧丽娟[1] CHEN Wen-ting;LI Zhong-jun;CHEN Jing-hua;ZENG Dai-di;HUANG Su-ran;XIAO Li-juan(Department of Gynecology and Obstetrics, Dongguan People's Hospital, Guangdong, Dongguan 523059,China)

机构地区：[1]东莞市人民医院妇产科

出　　处：《发育医学电子杂志》2019年第3期214-217,共4页Journal of Developmental Medicine (Electronic Version)

基　　金：国家卫生计生委医药卫生科技发展研究中心资助项目(W2016CWGD05)

摘　　要：目的 检测子痫前期孕妇胎盘组织中miR-205表达,并探讨其在子痫前期致病中的作用。方法 2017年10月至2018年7月,在东莞市人民医院就诊的子痫前期孕妇中,选择30例纳入本研究,选择同期在我院剖宫产分娩的30例产妇作为对照组。荧光定量PCR检测胎盘组织中miR-205、髓细胞白血病因子-1(myeloidcellleukemia-1,MCL-1)的表达水平。采用Western印迹法检测MCL-1蛋白表达。统计学方法采用独立样本t检验、Spearman线性相关分析法。结果 子痫前期组与对照组孕妇的年龄和分娩孕周比较,差异无统计学意义(P>0.05);两组的收缩压、舒张压和24h尿蛋白比较,差异有统计学意义(P<0.01)。子痫前期组孕妇胎盘组织中miR-205的2^-△△Ct值高于对照组(0.226±0.043与0.117±0.031,P=0.000),MCL-1低于对照组(0.162±0.033与0.273±0.048,P=0.000)。Spearman相关分析显示,子痫前期组孕妇胎盘组织中miR-205表达与MCL-1表达呈负相关(r=-0.326,P<0.05)。子痫前期组孕妇胎盘组织中MCL-1蛋白表达水平低于对照组(0.164±0.035与0.436±0.092,P<0.01)。结论 子痫前期患者胎盘组织中高表达的miR-205抑制MCL-1基因表达,可能参与子痫前期的致病过程。Objective To detect the mRNA-205 expression in placenta of pregnant woman with preeclampsia and explore its effect in the pathogenesis of preeclampsia. Methods From October 2017 to July 2018, 30 patients of preeclampsia were enrolled in Dongguan People's Hospital as well as 30 cases of normal delivery were selected as control group. Real-time PCR was used to detect the expression of miR- 205 in placenta and myeloid cell leukemia-1(MCL-1) mRNA. MCL-1 protein expression was detected by Western blot analysis. The statistical methods were carried out by independent sample t test and Spearman linear correlation analysis. Results There was no statistical significance in age and gestational week between preeclampsia group and control group (P>0.05). There was statistical significance in systolic blood pressure, diastolic blood pressure and 24-hour urinary protein between two groups (P<0.01). The 2^--△△ Ctt values of miR-205 in placenta of preeclampsia group was higher than that in control group [(0.226±0.043) vs (0.117±0.031), P=0.000]. MCL-1 in preeclampsia group was lower than that in control group[(0.162±0.033) vs (0.273±0.048), P=0.000]. Spearman linear correlation analysis showed the expression of mi R-205 in placenta of pregnant women for preeclampsia group was negatively correlated with MCL-1 (r=-0.326, P<0.05). The protein expression level of MCL-1 in placenta of pregnant women preeclampsia group was lower than that in control group[(0.164±0.035 ) vs (0.436±0.092), P<0.01]. Conclusion High expression of miR-205 in placenta of pregnant women for preeclampsia may inhibit the expression of MCL-1 to participate the pathogenesis of preeclampsia possibly.

关 键 词：miR-205 子痫前期 髓细胞白血病因子-1 胎盘 

分 类 号：R714.244[医药卫生—妇产科学]