Fe^0对污泥接种餐厨垃圾厌氧发酵及抗生素抗性基因的影响  

EFFECTS OF Fe^0 ON BIOGAS PRODUCTION AND THE FATE OF ANTIBIOTIC RESISTANCE GENES IN ANAEROBIC DIGESTION OF FOOD WASTE INOCULATED WITH SLUDGE

作　　者：王攀 杜晓璐 陈锡腾 孙淑婷 梁肖飞 任连海 吕峥 WANG Pan;DU Xiao-lu;CHEN Xi-teng;SUN Shu-ting;LIANG Xiao-fei;REN Lian-hai;LV Zheng(School of Food and Chemical Engineering,Beijing Technology and Business University,Beijing 100048,China;Department of Resource Conservation and Environmental Protection,National Development and Reform Commission,Beijing 100824,China)

机构地区：[1]北京工商大学食品学院,北京100048 [2]国家发展与改革委员会资源节约和环境保护司,北京100824

出　　处：《环境工程》2019年第7期178-182,共5页Environmental Engineering

基　　金：“十三五”时期北京市属高校高水平教师队伍建设支持计划(CIT&TCD201804016);国家自然科学基金(51578008)

摘　　要：在中温条件下以污泥为接种物,以餐厨垃圾为发酵底物,通过向厌氧发酵系统中投加不同量的Fe^0,考察其对餐厨垃圾厌氧发酵过程中日产气量、累积产气量、SCOD、氨氮、挥发性脂肪酸(VFAs)以及厌氧发酵体系中抗生素抗性基因行为特征的影响(包括β-内酰胺类 blaOXA-1、blaTEM,大环内脂类 ermB、ermF、mefA,磺胺类 sul1、sul2,四环素类 tetM、tetO、tetQ、tetW、tetX 和Ⅰ类整合子 intI1)。结果表明:投加Fe^0可以起到提高产气量,促进有机物的水解,缓解酸抑制的作用,当投加20 g/L Fe^0时产气量最高。由于经过湿热水解预处理,原始餐厨垃圾的抗性基因丰度较低,但接种污泥中 ermF、tetO、tetQ和tetW的丰度较高,分别为5.13×10^5,3.04×10^5,2.03×10^5,1.11×10^5 copies/g,经厌氧发酵后这4种抗性基因丰度削减明显,但是 mefA和tetM 的丰度与原始投加物料相比有所增长,投加20 g/L Fe^0与未投加Fe^0相比, mefA、tetM 2种抗性基因的丰度有一定削减。The anaerobic digestion of food waste was conducted under mesophilic conditions inoculated with wastewater treatment sludge.The influence of Fe^0 on the anaerobic fermentation performance,such as gas production,SCOD,ammonia-nitrogen,volatile fatty acids (VFAs) and the fate of antibiotic resistance genes (ARGs),was investigated.The ARGs include β-lactams ARGs (blaOXA-1,blaTEM),macrolides ARGs (ermB,ermF,mefA),sulfonamides ARGs (sul1,sul2),tetracycline ARGs (tetM,tetO,tetQ,tetW,tetX) and class I integron intI1.The results showed that the Fe^0 could increase the gas production,promote the hydrolysis of organics and alleviate the acid inhibition.When 20 g/L Fe^0 was added in the system,the gas production reached the maximum value.The abundance of ARGs in the original food waste was low due to the hydrothermal pretreatment,but the abundances of ermF,tetO,tetQ and tetW in the sludge were higher,detected as 5.13×10^5,3.04×10^5,2.03×10^5 and 1.11×10^5 copies/g,respectively.The abundance of these four ARGs was significantly reduced after anaerobic fermentation,but the abundance of mefA and tetM increased compared with the initial substrates.Compared with none Fe^0 group,the abundance of mefA and tetM reduced in the system after adding 20 g/L Fe^0.

关 键 词：餐厨垃圾 厌氧发酵 抗生素抗性基因 零价铁(Fe^0) 

分 类 号：X799.3[环境科学与工程—环境工程]