牵牛子水煎液对小鼠 H22肝癌皮下移植瘤的抗肿瘤作用和机制  

Anti-tumor effect and action mechanism of Semen Pharbitidis Decoction on H22 subcutaneously transplanted tumor in mice

作　　者：王梦琪 林海红[1] 杜钢军[1] WANG Mengqi;LIN Haihong;DU Gangjun(Intitute of Pharmacy, Pharmaceutical College of Henan University, Kaifeng 475004, China)

机构地区：[1]河南大学药学院药物研究所

出　　处：《河南大学学报（医学版）》2019年第2期77-84,共8页Journal of Henan University:Medical Science

基　　金：河南省自然科学基金项目(182300410310)

摘　　要：[目的]观察牵牛子水煎液对小鼠H22肝癌皮下移植瘤的抗肿瘤作用,分析其可能的抗肿瘤作用机制。[方法]建立小鼠H22肝癌皮下移植肿瘤模型,于肿瘤接种后次日进行随机分组。给药组用牵牛子水煎液4 g/kg进行15 d灌胃治疗,1次/d;模型组用生理盐水灌胃。通过小鼠体质量、自主活动、肿瘤体积、血常规等指标评价牵牛子水煎液对H22肝癌皮下移植瘤小鼠肿瘤生长的抑制作用,利用网络药理学分析牵牛子相关靶点和途径。[结果]模型组小鼠体质量显著上升,自主活动下降,中性粒细胞/淋巴细胞比值(NLR)、血小板/淋巴细胞比值(PLR)大;与模型组相比,给药组小鼠体质量增加减缓,自主活动增加,中性粒细胞/淋巴细胞比值(NLR)、血小板/淋巴细胞比值(PLR)小,肿瘤抑制率为33.69%(P<0.05)。网络药理学分析结果筛选出22个牵牛子有效活性成分,PPI网络鉴定了6个核心靶点,涉及30条KEGG通路和29个GO生物过程。[结论]牵牛子水煎液能够抑制小鼠H22肝癌皮下移植瘤的生长,其作用与22个牵牛子活性成分干预TNF信号通路、Toll样受体信号通路、FOXO信号通路、糖酵解或糖异生等途径相关。[Objective]To observe anti-tumor effect of Semen Pharbitidis decoction on H22 subcutaneously transplanted tumor in mice,and explore its possible action mechanism of action/mode of action.〔Methods〕The next day after the mouse model of H22 subcutaneously transplanted tumor was established,the tumor bearing mice were randomly divided into two groups;the treatment group and the control group.The mice were treated with Semen Pharbitidis decoction[4 g/kg per mouse] via intragastric administration once-daily for 15 days,the model group received physiological saline.Anti-tumor effect was evaluated by body weight,autonomic activities,tumor size and blood blood routine test.Network network pharmacology was used to analyze the related targets and pathways of Semen Pharbitidis.[Results] Compared with the control group,the Semen Pharbitidis-treated mice had slow body weight increase,increased autonomous activities,increased neutrophils vs lymphocytes and blood platelets vs lymphocytes rate.Compared with the model group,body weight was increased slowly,autonomic activities was increased,rate of neutrophils vs lympocytes and neutrophils vs Platelet was increased in treatment mice,The inhibitory rate of tumor growth was 33.69%(P<0.05).Network pharmacology analysis revealed 22 active components in Semen Pharbitidis,which regulate six core targets Involved in 30 KEGG pathway and 29 GO bioprocess.[Conclusion]Semen Pharbitidis decoction has an inhibitory effect on H22 subcutaneously transplanted tumor in mice,its mechanisms were associated with 22 active components Involved in TNF signaling pathway,Toll-like receptor signaling pathway,FOXO signaling pathway and glycolysis or gluconeogenesis.

关 键 词：牵牛子水煎液 网络药理学 活性成分 H22 肝癌 

分 类 号：R285.5[医药卫生—中药学]