早发型重度子痫前期患者血清2-ME水平和胎盘COMT表达及其意义  

Serum 2-Methoxyoestradiol levels and placental catechol-O-methyltransferase expression in early-onset severe pre-eclampsia and its significances

作　　者：黄丽琼[1] 郑波[1] 张元珍[2] HUANG Liqiong;ZHENG Bo;ZHANG Yuanzhen(Department of Gynaecology and Obstetrics,XianNing Central Hospital,the First AffiliatedHospital of Hubei University of Science and Technology,Xianning,Hubei 437000,China;Department of Gynaecology and Obstetrics,ZhongnanHospital of Wuhan University,Wuhan,Hubei 430071,China)

机构地区：[1]咸宁市中心医院湖北科技学院附属第一医院妇产科,湖北咸宁437000 [2]武汉大学中南医院妇产科,湖北武汉430071

出　　处：《国际检验医学杂志》2019年第14期1754-1758,共5页International Journal of Laboratory Medicine

基　　金：咸宁市科技局自然科学基金项目(201638)

摘　　要：目的 观察早发型重度子痫前期患者血清中2-甲氧雌二醇(2-ME)水平和胎盘组织儿茶酚-O-甲基转移酶(COMT)蛋白表达变化及其临床意义。方法 选取孕周为28~34周早发型重度子痫前期单胎孕妇25例;随机选取同期健康对照组25例,标准为孕周小于34周早产的单胎健康孕妇。收集两组年龄、孕周、孕产次、孕前体质量指数、采血日体质量指数、收缩压、舒张压、分娩孕周、期待治疗时间临床资料。采用酶联免疫吸附法检测两组孕妇血清2-ME水平,分析2-ME与临床指标的相关性。免疫组化检测两组胎盘组织中COMT的表达,逆转录-聚合酶链反应(RT-PCR)和蛋白质印迹法(Western Blot)法分别检测胎盘组织中COMT mRNA和蛋白的表达。结果 早发型重度子痫前期(EOSPE)组血清2-ME水平为(525.3±172.6)pg/mL,健康对照(NP)组为(705.9±157.4)pg/mL,EOSPE组血清2-ME水平低于NP组,差异有统计学意义( P <0.05);2-ME水平与收缩压呈负相关( r =-0.648, P <0.05);与期待治疗时间呈正相关( r =0.694, P <0.05)。两组孕妇胎盘组织中均有COMT表达,EOSPE组COMT mRNA相对表达量(0.447±0.152)与NP组COMT mRNA相对表达量(0.467±0.151)比较,差异无统计学意义( P >0.05);EOSPE组胎盘组织中COMT 蛋白相对表达量(0.891 6±0.102 0)与NP组(0.924 0±0.158 7)比较差异无统计学意义( P >0.05)。结论 早发型重度子痫前期患者血清2-ME水平降低,并与病情严重程度相关;胎盘COMT表达无明显变化。提示2-ME水平降低可能会导致子痫前期发生及发展,但与胎盘COMT表达无关。Objective To observe the levels of 2-methoxyestradiol (2-ME) in the serum and the protein expression changes and clinical significances of catechol-O-methyltransferase (COMT) in placenta tissue of patients with early-onset severe preeclampsia. Methods 25 cases with early-onset severe preeclampsia patients were selected (EOSPE group),who were singleton pregnancy and the gestational weeks were more than 28 weeks but less than 34 weeks;25 cases of healthy pregnant women were randomly selected as the normal control group (NP group),who were singleton pregnancy with gestational weeks less than 34 weeks and premature birth.The related clinical data were collected for a statistical analysis,such as age of the patients,gestational weeks,the gravidity and parity history,BMI before the pregnancy,BMI on the blood collection day,systolic blood pressure,diastolic blood pressure,gestational age,and expectant treatment time.The 2-ME levels in the serum of two groups were detected by ELISA,and the correlation between the 2-ME and clinical indicators was analyzed.The expression levels of catecholamine-o-methyltransferase (COMT) in placenta tissue of two groups were detected by the immunohistochemistry.The expression levels of COMT mRNA and protein in placenta tissue of two groups were separately detected by the methods of RT-PCR and Western Blot. Results The 2-ME level of pregnancy serum in the EOSPE group was (525.3±172.6) pg/mL,and the NP group was (705.9±157.4) pg/mL;The 2-ME level of pregnancy serum in EOSPE group was lower than those in NP group,and the differences were statistically significant ( P <0.05).There was a negative correlation between the 2-ME level and systolic blood pressure( r =-0.648, P <0.05);There was a positive correlation between the 2-ME level and the expectant treatment time( r =0.694, P <0.05.COMT expression was found in the placental tissues of pregnant women in the two groups.The relative expression of COMT mRNA in the EOSPE group (0.447±0.152) was compared with that in the NP group (0.467±0.151)

关 键 词：子痫前期 早发型 2-甲氧雌二醇 儿茶酚-O-甲基转移酶 

分 类 号：R714.244[医药卫生—妇产科学]