IFN-λ1作为乙型脑炎病毒DNA疫苗佐剂的免疫效应研究  

Immune effect of IFN-λ1 as adjuvant for Japanese encephalitis virus DNA vaccine

作　　者：周亚红 田占成[1] 于瑞明 高闪电[1] 独军政[1] 刘光远[1] 罗建勋[1] 殷宏[1,2] ZHOU Ya-hong;TIAN Zhan-cheng;YU Rui-ming;GAO Shan-dian;DU Jun-zheng;LIU Guang-yuan;LUO Jian-xun;YIN Hong(State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China.)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2019年第7期841-846,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31201899);中国农业科学院农业科技创新工程项目(2014ZL010)

摘　　要：为了评估IFN-λ1作为免疫佐剂能否提高乙型脑炎病毒DNA疫苗的免疫效应,利用RT-PCR方法扩增猪源IFN-λ1基因,构建重组真核表达载体pVAX1-IFN-λ1,通过Western-blot和间接免疫荧光试验鉴定免疫效果。结果表明,转染pVAX1-IFN-λ1重组质粒的BHK21细胞能够正确表达IFN-λ1;将重组IFN-λ1与pVAX1-prMEDNA疫苗免疫小鼠后,与对照组pVAX1-prME相比,IFN-λ1可以诱导小鼠产生乙型脑炎病毒EⅢ蛋白特异性抗体水平、乙脑病毒特异性中和抗体效价和淋巴细胞增殖水平显著提高。结论,重组猪源IFN-λ1作为乙型脑炎病毒DNA疫苗的分子免疫佐剂能够在小鼠动物模型上提高乙型脑炎病毒DNA疫苗的免疫效应。To evaluate whether IFN-λ1 as immune-adjuvant can improve the immune effect of Japanese encephalitis virus DNA vaccine,the recombinant pVAX1-IFN-λ1 plasmid was constructed after the porcine IFN-λ1 gene was amplified by RT-PCR.The immune effects were idendificated by Western-blot and indirect immunofluorescence assay.The results indicated that IFN-λ1 was accurately expressed in BHK21 cells transfected with the pVAX1-IFN-λ1 plasmid.Groups of mice were immunized with pVAX1-prME DNA vaccine and recombinant IFN-λ1,compared with the immunized group of pVAX1-prME DNA vaccine,IFN-λ1 can significantly enhance the induced anti-EⅢprotein specific antibody level,JEV-neutralizing antibody titers and the level of lymphocyte proliferation.In conclusion,typeⅢinterferon PoIFN-λ1 could enhance the immune responses of DNA vaccine of JEV in the mice model.

关 键 词：IFN-λ1 乙型脑炎病毒 DNA疫苗 鉴定 免疫 

分 类 号：S852.659.6[农业科学—基础兽医学]