早胜牛微卫星多态性及其与生长性状的关联分析  

Analysis of Microsatellite Polymorphism and Its Association with Growth Traits of Zaosheng Cattle

作　　者：迟浩斌 韩向敏[1] 郎侠[2] 王彩莲[2] CHI Haobin;HAN Xiangmin;LANG Xia;WANG Cailian(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]甘肃省农业科学院,兰州730070

出　　处：《中国畜牧兽医》2019年第7期1976-1985,共10页China Animal Husbandry & Veterinary Medicine

基　　金：甘肃省农业科学院博士基金:早胜牛种质资源评价及保护措施研究(2016GAAS27);甘肃省农业科学院农业科技创新专项学科团队项目:动物遗传育种与草食畜生产体系(2017GAAS30)

摘　　要：为了更好地了解早胜牛的遗传信息,为早胜牛的选育保种提供理论依据,本试验选取13对微卫星引物,应用PCR扩增和毛细管电泳的方法对48头早胜牛进行遗传多样性分析,并对13个位点不同基因型与早胜牛的初生重、6月龄重、成年体重、体高、体长、胸围和管围7个性状进行关联分析。结果显示,13个微卫星位点平均等位基因数目为9.54,平均有效等位基因数为4.792,平均观测杂合度为0.498,平均期望杂合度为0.783,平均多态信息含量为0.712。关联分析发现,与初生重存在关联的位点有D12S4和D15S10;与6月龄重存在关联的位点有D18S5和D12S4;与成年体重存在关联的位点有D14S31和D11S15;与体高存在关联的位点有D18S5、D15S10和D19S2;与体长存在关联的位点有D15S10、D14S31和D19S2;与胸围存在关联的位点有D18S5、D12S4和D19S2;与管围存在关联的位点有D18S5和D19S2。研究发现,选取的13个位点中存在关联的位点有6个:D18S5、D12S4、D15S10、D14S31、D19S2和D11S15。本试验所选微卫星位点在早胜牛群体中存在较丰富的多态性,并与经济性状具有关联性,可用于早胜牛遗传资源评价及早期选育改良。To better understand the genetic information and provide theoretical basis for the breeding and conservation of Zaosheng cattle,13 pairs of microsatellite primers were used to analyze the genetic diversity by PCR amplification and capillary electrophoresis in 48 Zaosheng cattle.The correlation between different genotypes at 13 microsatellite loci and seven traits(birth weight,6-month-old weight,adult weight,body height,body length,chest circumference and cannon circumference) of Zaosheng cattle were analyzed.The results showed that the average number of alleles at 13 microsatellite polymorphic loci was 9.54,the average effective allele was 4.792,the average observed heterozygosity was 0.498,the average expected heterozygosity was 0.783,and the average polymorphic information content was 0.712.The correlation analysis results showed that D12 S4 and D15 S10 were correlated with birth weight;D18 S5 and D12 S4 were correlated with 6-month-old weight;D14 S31 and D11 S15 were correlated with adult weight;D18 S5,D15 S10 and D19 S2 were correlated with body height;D15 S10,D14 S31 and D19 S2 were correlated with body length;D18 S5,D12 S4 and D19 S2 were correlated with chest circumference;D18 S5 and D19 S2 were correlated with cannon circumference.This results found that 6 loci(D18 S5,D12 S4,D15 S10,D14 S31,D19 S2 and D11 S15) were associated with the traits of Zaosheng cattle among the selected 13 microsatellite loci.The microsatellite loci selected in this experiment had rich polymorphisms in Zaosheng cattle population,and were associated with economic traits,which could be used for genetic resources evaluation and early breeding improvement of Zaosheng cattle.

关 键 词：微卫星标记 早胜牛 多态性 遗传标记 

分 类 号：S823.94[农业科学—畜牧学]