野生与种植黄连植株不同部位的生物碱含量比较研究  

Comparative Study on Alkaloid Content in Different Parts of Coptis chinensis Grown in Wild and Cultivated

作　　者：曾燕 朱俊平[1,3] 范诗琪 梁慧慧[1,3] 何红 张喜利 李龙 刘文龙[1,3] ZENG Yan;ZHU Junping;FAN Shiqi;LIANG Huihui;HE Hong;ZHANG Xili;LI Long;LIU Wenlong(School of Pharmacy College,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Department of Pharmacy,The Second Affiliated Hospital of Shaoyang Academy,Shaoyang,Hunan 422000,China;Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine,Changsha,Hunan 410208,China;Department of Pharmacy,Hunan Children's Hospital,Changsha,Hunan 410007,China)

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]邵阳学院附属第二医院药剂科,湖南邵阳422000 [3]中药成药性与制剂制备湖南省重点实验室,湖南长沙410208 [4]湖南省儿童医院药剂科,湖南长沙410007

出　　处：《湖南中医药大学学报》2019年第7期856-859,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金（81270055）;湖南省教育厅科研优秀青年项目（15B174）

摘　　要：目的采用UV和HPLC法比较野生与种植黄连植株不同部位生物碱含量差异。方法UV法在波长345nm处测定黄连总生物碱含量,HPLC采用C18(4.6mm×250mm,5μm)色谱柱,乙腈和0.4%磷酸水溶液(25∶75)等度洗脱,流速1.0mL/min,进样量10μL,检测波长345nm,柱温30℃以测定盐酸小檗碱含量。结果黄连不同部位的总生物碱含量:种植黄连根茎含(9.88±0.03)%、须根含(6.41±0.03)%、茎叶含(1.92±0.04)%;野生黄连根茎含(7.95±0.03)%、茎叶含(0.64±0.03)%。黄连不同部位的的盐酸小檗碱含量:种植黄连根茎含(0.45±0.04)%、须根含(0.17±0.03)%、茎叶含(0.09±0.03)%;野生黄连根茎含(0.38±0.03)%、茎叶含(0.16±0.03)%。结论种植黄连植株不同部位的总生物碱含量高于野生黄连,但其盐酸小檗碱含量低于野生黄连植株;野生与种植黄连均为根茎部总生物碱和盐酸小檗碱含量最高。Objective To compare the alkaloid content in different parts of wild and cultivated Coptis chinensis by UV and HPLC.Methods Determination of total alkaloids in Coptis chinensis by ultraviolet spectrophotometry at 345 nm.HPLC was performed on C18(4.6 mm × 250 mm, 5 μm) chromatographic column by isocratic elution with acetonitrile -0.4% phosphoric acid aqueous (25:75) as mobile phase, the flow rate was 1 mL/min, the injection volume was 10 μL, the detection wave length was 345 nm, the column temperature was set at 30 ℃.Results The content of total alkaloids in cultivated Coptis chinensis: the rhizome, fibrous root, cauline contains (9.88±0.03)%,(6.41±0.03)%,(1.92±0.04)%, respectively;The content of total alkaloids in wild Coptis chinensis: rhizome was (7.95±0.03)%, stem and leaf was (0.64±0.03)%.The content of berberine hydrochloride in cultivated Coptis chinensis: rhizome was (0.45±0.04)%, fibrous root, and stem and leaf contain (0.17±0.03)%,(0.09±0.03)%, respectively.The content of berberine hydrochloride in wild Coptis chinensis: rhizome was (0.38±0.03)%, and that of stem and leaf was (0.16±0.03)%. Conclusion The content of total alkaloids in different parts of cultivated Coptis chinensis was higher than that in wild Coptis chinensis, but the content of berberine hydrochloride was lower than that in wild Coptis chinensis. The rhizome part in both wild and cultivated Coptis chinensis had the highest content of total alkaloids and berberine hydrochloride.

关 键 词：黄连 UV HPLC 盐酸小檗碱 生物碱标志物 

分 类 号：R284.1[医药卫生—中药学]