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作 者:李清 何明月 瞿发林 谭兴起 董文燊 倪玉佳 邵留英 LI Qing;HE Mingyue;QU Falin;TAN Xingqi;DONG Wenshen;NI Yujia;SHAO Liuying(Hospital 904 of Joint Logistic Support Force of PLA,Changzhou 213003,China)
出 处:《药学实践杂志》2019年第4期348-351,共4页Journal of Pharmaceutical Practice
基 金:原南京军区重大专项课题(15DX005)
摘 要:目的 采用高效液相色谱(HPLC)法测定不同产地假蒟中α-细辛脑的含量。方法色谱柱为Lichrospher C 18 柱(150 mm×4.6 mm,5 μm),流动相为乙腈-0.05%磷酸溶液(47:53),流速为1.0 ml/min,检测波长313 nm,柱温30 ℃。结果α-细辛脑在0.0970~1.934 μg范围内与峰面积有良好线性关系( r =0.999 8),平均回收率为99.94%(RSD=1.13%),样品在24 h内稳定,且该方法重复性较好。结论 本方法简便准确,可用于假蒟药材中α-细辛脑的含量测定,以控制其质量;我国不同产地的假蒟中α-细辛脑含量有明显差异,其中以广西产假蒟含量最高。Objective To determine the content of α-Asarone in Piper sarmentosum Roxb.from different habitats by HPLC. Methods The chromatographic column was Lichrospher C 18 (150 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.05% H 3 PO 4 solution (47:53) with a flow rate of 1.0 ml·min -1 ,the UV detection wavelength at 313 nm and the column temperature at 30 ℃. Results There was a good linear relationship for α-Asarone in the range of 0.097 0~1.934 μg (r=0.999 8).The average recovery was 99.94%(RSD=1.13%).The sample was stable within 24 h.The method had good repeatability. Conclusions This method is simple and accurate for the content assay of α-Asarone in Piper sarmentosum Roxb.It can be used to control the quality of Piper sarmentosum Roxb.There was a significant difference of α-Asarone content in Piper sarmentosum Roxb.from different provinces in China.The Piper sarmentosum Roxb.from Guangxi province has the highest content of α-Asarone .
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