Production of Pectinolytic Enzymes by Two Bacillus spp. Strains and Their Application in Flax Degumming  

作　　者：Jingping Ge Zhiyu Yang Renpeng Du Li Zhang Wenxiang Ping Dan Zhao 

机构地区：[1]Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China [2]Key Laboratory of Microbiology,School of Life Sciences,Heilongjiang University,Harbin 150080,China [3]School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China

出　　处：《Transactions of Tianjin University》2019年第4期413-419,共7页天津大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 31270534, 31770538);the National Natural Science Youth Foundation of China (No. 31300355);the Post-Doctorate Foundation of Heilongjiang Province (No. LBH-Z15214)

摘　　要：This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these fi ndings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were signifi cantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out effi ciently and quickly. The mixed system exhibits feasible applications in the fi ber and textile industry.This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of flax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these findings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were significantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out efficiently and quickly. The mixed system exhibits feasible applications in the fiber and textile industry.

关 键 词：FLAX DEGUMMING RETTING PECTIN Pectate Pectinolytic enzyme Fiber 

分 类 号：T[一般工业技术]