RP-HPLC法测定利鼻片中绿原酸和咖啡酸的含量  

Determination of Chlorogenic Acid and Caffeic Acid in Libi Tablets by RP-HPLC

作　　者：邱颖 Qiu Ying(Huangshan Vocational and Technical College,Huangshan 245000 China)

机构地区：[1]黄山职业技术学院

出　　处：《锦州医科大学学报》2019年第4期1-3,I0001,共4页Journal of Jinzhou Medical University

摘　　要：目的建立反相高效液相色谱法测定利鼻片中绿原酸和咖啡酸的含量。方法以利鼻片为研究对象,采用GeminiC18色谱柱(4.6mm×250mm,5μm),以甲醇-0.1%磷酸水溶液(20∶80;v/v)为流动相;流速:1.0mL/min;检测波长:323nm;柱温:35℃。结果绿原酸和咖啡酸分别在1.025~10.25μg/mL(r=0.9992,n=6);0.8~8.0μg/mL(r=0.9998,n=6)浓度范围内与峰面积呈良好线性关系;平均加样回收率(n=9)分别为98.1%(RSD=1.8%)和99.4%(RSD=0.99%)。结论本测定方法简便、快捷、准确,为测定利鼻片中绿原酸和咖啡酸的含量提供了准确可靠的分析方法。Objective To use RP-HPIC for the determination of Chlorogenic acid and Caffeic acid in Libi tablets.Methods Libi tablets were taken as the research object.The samples were analyzed in Gemini C 18 column (4.6 mm × 250 mm,5 μm),and the mobile phase was composed of methanol and 0.1% phosphoric acid solution(20∶80,v/v)at a flow rate of 1.0 mL/min.The UV 323 nm was set as the detection wavelength,and the column temperature was 35 ℃.Results The calibration curve was linear within the range of 1.025 to 10.25 μg/mL( r =0.999 2, n =6) for Chlorogenic acid,and 0.8 to 8.0 μg/mL( r =0.999 8, n =6) for Caffeic acid.The average recovery was 98.1%(RSD=1.8%) for Chlorogenic acid,and 99.4%(RSD=0.99%) for Caffeic acid.Conclusion The method proves to be simple,fast,accurate and reliable in the determination of Chlorogenic acid and Caffeic acid in Libi tablets.

关 键 词：利鼻片 绿原酸 咖啡酸 反相高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]