新生大鼠七氟醚麻醉后海马α7nAChR、AChE和ChAT表达的变化  

Changes in expression of hippocampalα7nAChR,AChE and ChAT after sevoflurane anesthesia in neonatal rats

作　　者：杨洋 钟远平 赵鹏程 张超 朱宇航 林夏妃 朱昭琼 Yang Yang;Zhong Yuanping;Zhao Pengcheng;Zhang Chao;Zhu Yuhang;Lin Xiafei;Zhu Zhaoqiong(Key Laboratory of Fundamental Research on Anesthesiology and Organ Protection of Guizhou Provincial,Zunyi Medical University,Zunyi 563000,China;Department of Anesthesiology,Affiliated Hospital of Zunyi Medical University,Zunyi 563099,China)

机构地区：[1]遵义医科大学贵州省麻醉与器官保护基础研究重点实验室,563000 [2]遵义医科大学附属医院麻醉科,563000

出　　处：《中华麻醉学杂志》2019年第3期279-282,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81360180,81660193).

摘　　要：目的评价新生大鼠七氟醚麻醉后海马α7烟碱型乙酰胆碱受体(α7nAChR)、胆碱酯酶(AChE)和乙酰转移酶(ChAT)表达的变化.方法健康SD大鼠72只,7日龄,体重25~40g,雌雄不拘,采用随机数字表法分为3组(n=24):对照组(C组)、空氧组(A∕O组)和七氟醚组(S组).A∕O组吸入运载气体(1L∕min空气+1L∕minO2)2h,S组吸入3.4%七氟醚+运载气体2h.于吸入结束后2h、1周及4周各随机选取8只大鼠,处死后取海马组织,分别采用Westernblot法和RT-PCR法检测α7nAChR、AChE、ChAT及其mRNA的表达水平.结果与A∕O组比较,S组吸入结束后各时点α7nAChRmRNA表达下调,α7nAChR吸入结束后2h时表达下调,1周时表达上调;AChEmRNA吸入结束后2h时表达上调,4周时表达下调,吸入结束后4周时AChE表达下调;吸入结束后2h时ChATmRNA表达上调,吸入结束后各时点ChAT表达下调(P<0.05).结论新生大鼠七氟醚麻醉后海马α7nAChR表达出现先下调后上调趋势,ChAT和晚期AchE表达下调;上述蛋白表达趋势与其mRNA表达趋势有差异,提示七氟醚可能通过其他途径影响蛋白的表达.Objective To evaluate the changes in the expression of hippocampalα7 nicotinic acetylcholine receptor(α7nAChR),acetylcholinesterase(AChE)and choline acetyltransferase(ChAT)after sevoflurane anesthesia in neonatal rats.Methods Seventy-two healthy Sprague-Dawley rats of both sexes,aged 7 days,weighing 25-40 g,were divided into 3 groups(n=24 each)using a random number table method:control group(group C),air and oxygen group(group A/O)and sevoflurane group(group S).Rats were exposed to carrier gas(air 1 L/min plus oxygen 1 L/min)for 2 h in group A/O.Rats were exposed to 3.4%sevoflurane in carrier gas for 2 h in group S.Eight rats in each group were selected at 2 h,1 week and 4 weeks after the end of inhalation,and sacrificed,brains were removed and hippocampal tissues were obtained for determination ofα7nAChR,AChE and ChAT protein and mRNA by Western blot and real-time polymerase chain reaction,respectively.Results Compared with group A/O,the expression ofα7nAChR mRNA was significantly down-regulated at each time point after the end of inhalation,and the expression of TnAChR was down-regulated at 2 h after the end of inhalation and up-regulated at 1 week after the end of inhalation,the expression of AChE mRNA was up-regulated at 2 h after the end of inhalation and down-regulated at 4 weeks after the end of inhalation,the expression of AChE was down-regulated at 4 weeks after the end of inhalation,the expression of ChAT mRNA was up-regulated at 2 h after the end of inhalation,and the expression of ChAT was down-regulated at each time point after the end of inhalation in group S(P<0.05).Conclusion The expression of hippocampalα7nAChR is down-regulated at first and then up-regulated after sevoflurane anesthesia,the expression of ChAT and AchE in the later period is down-regulated,the tendency of protein expression mentioned above is different from that of its mRNA expression,suggesting that sevoflurane may affect the protein expression through other pathways.

关 键 词：婴儿 新生 麻醉药 吸入 受体 胆碱能 乙酰胆碱酯酶 胆碱O-乙酰转移酶 

分 类 号：R614[医药卫生—麻醉学]