芝麻素对氟胁迫下斑马鱼头部抗氧化功能和免疫相关酶活性的影响  

Effect of sesamin on the oxidative stress and activity of immune-related enzymes in zebrafish after exposure to fluoride

作　　者：王天玉 郭文静 曹谨玲 陈剑杰 WANG Tian-yu;GUO Wen-jing;CAO Jin-ling

机构地区：[1]山西农业大学动物科技学院山西省生态畜牧与环境兽医学重点实验室

出　　处：《饲料研究》2019年第6期22-26,共5页Feed Research

基　　金：国家自然科学基金(项目编号:31440087、31502141);中国博士后科学基金项目(项目编号:2012M520601、2013T60267)

摘　　要：为了探讨芝麻素对氟胁迫下斑马鱼头部抗氧化功能和非特异性免疫的影响,采用半静态法,将驯养后的斑马鱼,随机分为对照组(CK)、1 g/kg芝麻素组(S1)、2 g/kg芝麻素组(S2),80 mg/L氟胁迫组(F)、1 g/kg芝麻素组+80 mg/L氟胁迫组(FS1)、2 g/kg芝麻素组+80 mg/L氟胁迫组(FS2),分别在试验进行45、90 d时取斑马鱼头部,并对头部组织中的活性氧(ROS)、超氧化物歧化酶(SOD)、丙二醛(MDA)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)进行测定。结果表明,与F组比较,饲粮中添加芝麻素的FS1和FS2组斑马鱼头部ROS、MDA含量明显下降(P<0.05),而SOD活性明显上升(P<0.05),免疫相关酶ACP和AKP活性明显上升(P<0.05)。由此可见,饵料中添加一定剂量的芝麻素后,可以在一定程度上缓解氟胁迫对斑马鱼头部造成的氧化应激和免疫毒性作用。In order to investigate the effect of sesamin on the antioxidant function and non-specific immunity of head in zebrafish exposed to fluoride,zebrafishes were randomly divided into control group(CK),1 g/kg sesamin group(S1),2 g/kg sesamin group(S2),80 mg/L fluoride group(F),1 g/kg sesamin+80 mg/L fluoride group(FS1),and 2 g/kg sesamin+80 mg/L fluoride group(FS2),respectively.After exposure for 45 and 90 days,the reactive oxygen species(ROS),superoxide dismutase(SOD),malondialdehyde(MDA),alkaline phosphatase(AKP)and acid phosphatase(ACP)in the head of zebrafish were measured.The results showed that ROS and MDA in FS1 and FS2 groups were significantly decreased compared with the F group(P<0.05),while SOD activity,the activities of ACP and AKP in FS1 and FS2 groups was remarkably increased compared with the F group(P<0.05).All above results demonstrated that the addition of a certain dose of sesamin could alleviate the oxidative stress and immunotoxicity of head in zebrafish caused by fluoride to some extent.

关 键 词：芝麻素 氟 斑马鱼 氧化应激 免疫 

分 类 号：S965[农业科学—水产养殖]