2017年北京市首起人博卡病毒聚集性疫情流行病学调查和分析  

Epidemiologic investigation to the first cluster of fever cases caused by human bocavirus in Beijing, 2017

作　　者：董晓根[1] 张志敏 秦萌[1] 李若曦[1] 封会茹[1] 冯宝玉 余红[1] 尉秀霞[1] 王兆娥[1] 石婧 张建军[1] Dong Xiaogen;Zhang Zhimin;Qin Meng;Li Ruoxi;Feng Huiru;Feng Baoyu;Yu Hong;Wei Xiuxia;Wang Zhaoe;Shi Jing;Zhang Jianjun(Fengtai District Center for Disease Control and Prevention,Beijing 100071,China)

机构地区：[1]北京市丰台区疾病预防控制中心

出　　处：《疾病监测》2019年第6期506-510,共5页Disease Surveillance

基　　金：国家科技重大专项(No.2017ZX10103004)~~

摘　　要：目的对一起由人博卡病毒(HBoV)引起的聚集性发热疫情进行调查分析。了解其流行情况及HBoV毒株的进化情况,为今后HBoV发热疫情的防治提供参考依据。方法对2017年北京市丰台区13例发热患儿展开流行病学调查,采集咽拭子标本,并采用实时荧光PCR法进行呼吸道病毒核酸检测。对HBoV阳性标本进行VP1基因区特异性扩增和测序,通过DNAStar7.1、Bioedit v7.0.9、MEGA 6.0.6等生物信息学软件对扩增序列进行基因离散率和系统进化树分析。结果发病患儿临床症状以发热、咳嗽等呼吸道症状为主。患儿具有明显聚集性。10份样本中共检出3份HBoV阳性。基因离散率和进化树显示,3份样本均为HBoV-1型,核苷酸序列同源性100%,与北京株311-BJ07等流行株高度同源。结论此次聚集性发热疫情是由1型HBoV引起的,证实该型HBoV已在我国广泛存在。提示HBoV-1型引起的呼吸道感染形势严峻,应加强关注和研究,制定防治措施。Objective To understand the etiologic and epidemiologic characteristics of a cluster of human bocavirus(HBoV)infection cases in Fengtai district of Beijing and the circulation and evolution of HBoV in Beijing,and provide scientific evidence for the prevention and control of HBoV infection.Methods Epidemiologic investigation was performed for 13 children with fever,and 10 throat swabs were collected from them.The pathogens were identified by the multiplex combined real-time PCR detection kit for respiratory viruses.The HBoV VP1 gene region was specifically amplified and sequenced for the detected HBoV strains.The gene distance calculation and phylogenetic tree analysis were performed for the amplified sequences by using bioinformatics software DNAStar 7.1,Bioedit v7.0.9,and MEGA 6.0.6.Results The clinical symptoms of the children were mainly fever and cough.All the children with fever were classmates.Three HBoV positive samples were detected in 10 throat swabs,and the 3 HBoV strains were classified into type 1 indicated by the gene distance and phylogenetic tree analysis.The nucleotide sequences of the 3 HBoV strains shared 100% homology and the 3 strains were highly homologous to Beijing strain 311-BJ07,suggesting that the outbreak may be related to the circulating HBoV-1 strain in Beijing.Conclusion This cluster of fever cases was caused by HBoV-1 strain which are widespread in China.It suggested that HBoV-1 respiratory infection epidemic is serious.It is necessary to strengthen surveillance and research and develop effective HBoV-1 infection prevention and control measures.

关 键 词：人博卡病毒 聚集性发热疫情 序列分析 

分 类 号：R373[医药卫生—病原生物学] R181.3[医药卫生—基础医学]