基于黑磷量子点的光热效应在树突状细胞激活中的作用  

The black phosphorus quantum dots-based photothermal effect on dendritic cells activation

作　　者：叶鑫宇 梅林[2,3] YE Xin-yu;MEI Lin(School of Life Sciences,Tsinghua University,Beijing 100084,China;Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China;School of Pharmaceutical Sciences(Shenzhen),Sun Yat-Sen University,Shenzhen 510275,China)

机构地区：[1]清华大学生命科学学院,北京100084 [2]清华大学深圳研究生院,广东深圳518055 [3]中山大学药学院·深圳,广东深圳510275

出　　处：《药学学报》2019年第7期1297-1302,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(90612002,90612002)

摘　　要：癌症的免疫治疗是近年来兴起的一种治疗癌症的方法,通过人为地激活免疫系统来产生持久地杀伤肿瘤细胞的免疫反应,肿瘤疫苗是其最发达的部分之一。虽然目前肿瘤疫苗已经取得了很多突破,但仍然面临着巨大的挑战。在本研究中,采用癌细胞膜囊泡(cancer cell membrane nanovesicle,CCNVs)包裹黑磷量子点(black phosphorus quantum dots,BPQDs)制备得到负载黑磷量子点的癌细胞膜囊泡(BPQD-CCNVs),并将从小鼠骨髓中分离得到的树突状细胞与BPQD-CCNVs共同孵育,然后用808 nm近红外光对培养基照射10 min,最后用流式细胞分析仪检测树突状细胞表面分子CD80、CD86和MHC-II的表达情况。所有动物实验均经过清华大学动物伦理委员会批准。结果表明,基于黑磷量子点的光热效应能使培养基的温度升高,刺激树突状细胞成熟,使树突状细胞表面CD80、CD86和MHC-II的表达上调。本研究证明光热作用可以刺激树突状细胞的成熟。因此,可将光热作用引入到肿瘤疫苗中,增强肿瘤疫苗激活免疫系统的能力。Immunotherapy is the most active research area for cancer treatment.Tumor vaccine is one of the most developed aspects of cancer immunotherapy.Though tumor vaccine has made many breakthrough,it still faces many challenges.In this study,we coated the black phosphorus quantum dots(BPQDs) with cancer cell membrane to create a nanoparticle named BPQD-CCNVs.The BPQD-CCNVs were incubated with bone marrowderived dendritic cells and irradiated with 808 nm infrared light.We tested the expression level of CD80,CD86 and MHC II of dendritic cells by flow cytometry after irradiation.All animal experiments approved by the Animal Experiments Ethical Committee of Tsinghua University.The results showed that the rise of medium’s temperature caused by the photothermal effect of BPQDs could upregulate the expression of CD80,CD86 and MHC-II on dendritic cell surface.Based on these,we conclude that near infrared irradiation can stimulate the activation of dendritic cells.Our study may have provided a new strategy for tumor vaccine development.

关 键 词：免疫治疗 肿瘤疫苗 黑磷量子点 细胞膜 树突状细胞 

分 类 号：R943[医药卫生—药剂学]