丹参多酚酸治疗局灶性脑缺血再灌注损伤大鼠对神经行为及因子水平的影响  

Effects of Salvia miltiorrhiza on cerebral infarction volume, nerve behavior and SOD, GSH-px, MDA, BDNF and GDNF in rats

作　　者：孙瑞坦[1] 朴翔宇[1] 蔡鸣[1] 李研[1] 罗斯文 SUN Rui-tan;PIAO Xiang-yu;CAI Ming;LI Yan;Luo Si-wen(Department of Neurology,Zhongshan Hospital,Dalian University,Dalian 116001,China)

机构地区：[1]大连大学附属中山医院神经内科

出　　处：《海南医学院学报》2019年第14期1057-1060,共4页Journal of Hainan Medical University

基　　金：大连市医学科学研究计划项目(1811105)~~

摘　　要：目的:探讨丹参多酚酸治疗局灶性脑缺血再灌注损伤大鼠对脑梗死体积、神经行为及脑组织中过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、脑源性神经营养因子(BDNF)、胶质源性神经营养因子(GDNF)的影响。方法:选取健康成年雄性SD大鼠(清洁级)80只,采用随机数字表法分为假手术组(等量生理盐水)、模型组(等量生理盐水)、低剂量组(10mg/kg丹参多酚酸)、高剂量组(30mg/kg丹参多酚酸)、依达拉奉组(6mg/kg)各16只,对比各组大鼠术后24、48、72h的神经行为学评分、术后24h的脑梗死体积;测定各组大鼠72h后的脑组织中SOD、GSH-Px等指标。结果:术后24h,低剂量组、高剂量组和依达拉奉组的脑梗死体积均小于模型组(P<0.05),高剂量组和依达拉奉组的脑梗死体积均小于低剂量组(P<0.05);术后24、48、72h,低剂量组、高剂量组和依达拉奉组的神经行为学评分均小于模型组(P<0.05),高剂量组和依达拉奉组的神经行为学评分均小于低剂量组(P<0.05);低剂量组、高剂量组和依达拉奉组的脑组织中SOD、GSH-Px、BDNF、GDNF均高于模型组(P<0.05),而MDA低于模型组(P<0.05);高剂量组和依达拉奉组的脑组织中SOD、GSH-Px、BDNF、GDNF均高于低剂量组(P<0.05),而MDA低于低剂量组(P<0.05)。结论:丹参多酚酸治疗局灶性脑缺血再灌注损伤大鼠能促进氧自由基的清除,抑制脂质过氧化,从而减少梗死面积,达治疗效果。Objective:To investigate the effects of Salvia miltiorrhiza on cerebral infarction volume,nerve behavior and brain tissue SOD,GSH-Px,MDA,GDNF,BDNF in rats with focal cerebral ischemia reperfusion injury.Methods:Healthy adult male SD rats(clean grade)80,were randomly divided into sham operation group(saline),model group(saline),low dose group(10 mg/kg salvianolate),high dose group(30 mg/kg salvianolate)and edaravone group(6 mg/kg)with 16 rats in each groups were compared after operation in rats,48 h neural behavior of 24 h and 72 h scores,cerebral infarction volume 24 h after operation;SOD,GSH-Px and other indicators of brain tissue of rats after the determination of 72 h.Results:After 24 h,low dose group,high dose group and edaravone group,the cerebral infarction volume were less than the model group(P<0.05),high dose group and edaravone group,the infarct volume was less than the low dose group(P<0.05);after 24 h,48 h,72 h,scores of neurological behavior in the low dose group and high dose group and Yidala in the study group were lower than model group(P<0.05),neural behavior in high dose group and edaravone group scores were lower than the low dose group(P<0.05);low dose group,high dose group and edaravone group in the brain tissue of SOD,GSH-Px,BDNF,GDNF were higher than that of model group(P<0.05),and MDA was lower than that of model group(P<0.05);high dose group and edaravone group in the brain tissue of SOD,GSH-Px,BDNF,GDNF were higher than those in the low dose group(P<0.05),and MDA was lower than that of low dose group(P<0.05).Conclusion:The treatment of focal cerebral ischemia reperfusion injury in rats by inhibiting lipid peroxidation,protecting Magnesium Oxide's activity,thereby reaching the therapeutic effect.

关 键 词：丹参多酚酸 局灶性 脑缺血 再灌注损伤 

分 类 号：R285.5[医药卫生—中药学]