西藏色季拉山毛壳属及形态相似属真菌的多样性及系统发育分析  

Diversity and phylogenetic analysis of Chaetomiumand Chaetomium-like fungi from Sejila Mountains in Tibet

作　　者：岳海梅 潘朝晖 YUE Haimei;PAN Zhaohui(2.Research Institute of Tibet Plateau Ecology,Linzhi 860000,China)

机构地区：[1]西藏农牧学院植物科学学院,西藏林芝860000 [2]西藏高原生态研究所,西藏林芝860000

出　　处：《厦门大学学报（自然科学版）》2019年第4期532-542,共11页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(31360006,31760006);西藏自治区自然科学基金(XZ 2018ZR G-21)

摘　　要：从西藏色季拉山不同海拔采集的323份标本中分离毛壳属(Chaetomium)及形态相似属真菌,共分离到29个菌株,通过形态学特征将其中的27个菌株分别鉴定为3属5种:球毛壳(C.globosum)、马德拉斯毛壳(C.madrasense)、近缘毛壳(C.subaffine)、绳生二叉毛壳(Dichotomopilus funicola)和粗壮围领毛壳(Colariella robusta).通过ITS rDNA和28SrDNA D1/D2区序列构建系统发育树,分离的29个菌株被划分为6个种.碱基变异分析结果表明,ITS rDNA序列较28SrDNA D1/D2区序列变异更大,结合这两段基因序列可进行毛壳属及形态相似属真菌属内种间的系统发育研究.这些结果可丰富西藏毛壳属及形态相似属真菌资源库,为开发毛壳属及形态相似属真菌代谢产物奠定基础.323 samples were collected from different altitudes of Sejila Mountains in Tibet,and 29 strains of Chaetomium and Chaetomium-like fungi were isolated.The results showed that 27 strains of them were identified as 3 genera 5 species,C.globosum,C.madrasense,C.subaffine,Dichotomopilus funicola and Colariella robusta.Phylogenetic tree was constructed based on sequences of ITS rDNA and 28S rDNA D1/D2 region,showing that the 29 isolated strains were divided into 6 groups.The results of base mutation analysis showed that the ITS rDNA region had more variations than that of the 28S rDNA D1/D2 region,and the combination with these two gene sequences could be used in the phylogenetic study between species within genus of Chaetomium and Chaetomium-like fungi.Taken together,this study enriched the resources of Chaetomium and Chaetomium-like fungi in Tibet and laid a foundation for exploring metabolites of Chaetomium and Chaetomium-like fungi.

关 键 词：色季拉山 毛壳属 真菌 系统发育分析 

分 类 号：Q939.5[生物学—微生物学]