拟赤梢鱼消化酶和免疫酶的分布与活性  

Distribution and Activity of Digestive and Immune Enzymes in Pseudaspius leptocephalus

作　　者：金建丽[1] 李伟航[1,2] 陆灏 刘泓玮 JIN Jianli;LI Weihang;LU Hao;LIU Hongwei(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157011;Mudanjiang Medical School,Mudanjiang,Heilongjiang 157011,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]牡丹江市卫生学校,黑龙江牡丹江157011

出　　处：《贵州农业科学》2019年第7期75-79,共5页Guizhou Agricultural Sciences

基　　金：牡丹江师范学院研究生科技创新项目（kjcx2018-03mdjnu）

摘　　要：为拟赤梢鱼基础生物学及消化生理特征的深入研究奠定理论基础,采用聚丙烯酰胺凝胶电泳(PAGE)法,分别研究拟赤梢鱼消化系统(食道、前肠、中肠、后肠和肝胰脏)中蛋白酶(PRO)、淀粉酶(AMS)、酯酶(EST)3种消化酶和超氧化物歧化酶(SOD)、过氧化物酶(POD)2种免疫酶的分布及活性。结果表明:消化酶和免疫酶在拟赤梢鱼的消化系统中均有表达,并存在谱带缺失现象;PRO活性为食道>前肠>中肠>后肠>肝胰脏,AMS活性为后肠>中肠>前肠>食道>肝胰脏,EST活性为肝胰脏>前肠>中肠>食道>后肠,SOD活性为前肠>中肠>食道>肝胰脏>后肠,POD活性为肝胰脏>后肠>中肠>食道>前肠,各种酶在消化系统中分布及活性均表现出明显的组织特异性。The distribution and activity of PRO,AMS,EST,SOD and POD in digestive system of Pseudaspius leptocephalus were analyzed by PAGE to lay the theoretical foundation for intensive study of basic biology and digestive physiological characteristics of P.leptocephalus.Result:The digestive and immune enzymes all express in esophagus,foregut,midgut,hindgut and hepatopancreas of P.leptocephalus and there are phenomena of band loss.The PRO activity is esophagus>foregut>midgut>hindgut>hepatopancreas.The AMS activity is hindgut>midgut>foregut>esophagu>hepatopancreas.The EST activity is hepatopancreas>foregut>midgut>esophagus>hindgut.The SOD activity is foregut>midgut>esophagus>hepatopancreas>hindgut.The POD activity is hepatopancreas>hindgut>midgut>esophagus>foregut.The distribution and activity of five enzymes in digestive system express obvious tissue specificity.

关 键 词：拟赤梢鱼 消化系统 消化酶 免疫酶 电泳 

分 类 号：S917[农业科学—水产科学]