桃红四物汤促进产后血瘀大鼠子宫修复的作用  

Efficacy of Taohong Siwu Decoction in Promoting Uterine Repair in Rats with Postpartum Blood Stasis

作　　者：张琪 李白坤[2] 朱继民[2] 刘竹青[3] 苏婧婧 李庆林 ZHANG Qi;LI Bai-kun;ZHU Ji-min;LIU Zhu-qing;SU Jing-jing;LI Qing-lin(School of Pharmacy, Anhui University of Chinese Medicine, Anhui Hefei 230012, China;School of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Anhui Hefei 230012, China;College of Acupuncture and Moxibustion, Anhui University of Chinese Medicine, Anhui Hefei 230012, China;Experimental Research Center, Anhui University of Chinese Medicine, Anhui Hefei 230038, China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽中医药大学中西医结合学院,安徽合肥230012 [3]安徽中医药大学针灸推拿学院,安徽合肥230012 [4]安徽中医药大学科研实验中心,安徽合肥230038

出　　处：《安徽中医药大学学报》2019年第4期72-75,共4页Journal of Anhui University of Chinese Medicine

基　　金：安徽省高等学校省级自然科学研究项目(KJ2019A0460);国家自然科学基金项目(81673650)

摘　　要：目的 观察桃红四物汤对产后血瘀大鼠子宫修复的促进作用,探讨其与内皮素-1(endothelin-1,ET-1)、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)及血管内皮生长因子(vascular endothelial growth factor,VEGF)和血管内皮生长因子受体2(vascular endothelial growth factor receptor2,VEGFR -2)的关联性。方法 选取产后血瘀模型复制成功的大鼠40只,将其随机分为桃红四物汤高剂量(18.0 g/kg)、中剂量(9.0 g/kg)、低剂量(4.5 g/kg)组,阳性对照组(益母草颗粒4.3 g/kg)和模型组(蒸馏水),另取正常雌性大鼠作为正常对照组。连续给药7 d后,腹主动脉取血,分离子宫,苏木精-伊红染色法观察子宫血瘀状态,免疫组织化学法检测子宫VEGFR-2表达情况;酶联免疫吸附法检测血清中ET-1、iNOS及VEGF水平。结果 桃红四物汤可明显改善子宫血瘀程度,促进子宫组织中VEGFR-2的表达;桃红四物汤高、中剂量组大鼠血清VEGF及iNOS水平明显升高(P<0.05), ET-1水平明显降低(P<0.05)。结论 桃红四物汤可促进产后血瘀大鼠子宫修复,其修复作用可能与调节子宫血管舒缩功能及促进子宫血管新生有关。Objective To observe the efficacy of Taohong Siwu Decoction in promoting uterine repair in rats with postpartum blood stasis, and to investigate its effects on endothelin-1 (ET-1), inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), and vascular endothelial growth factor receptor2 (VEGFR-2). Methods Forty rats in which a model of postpartum blood stasis was successfully established were randomly divided into high-, middle-, and low-dose Taohong Siwu groups (18.0 g/kg , 9.0 g/kg, and 4.5 g/kg), positive control group (Herba Leonuri Granule 4.3 g/kg), and model group (distilled water);normal female rats were selected as normal control group. All rats received respective treatments for 7 consecutive days. Blood was collected from the abdominal aorta, and the uterus was isolated. HE staining was used to evaluate blood stasis in the uterus. The expression of VEGFR-2 in the uterus was measured by immunohistochemistry. Serum levels of ET-1, iNOS, and VEGF were determined by ELISA. Results Taohong Siwu Decoction significantly relieved blood stasis in the uterus and increased the expression of VEGFR-2 in the uterus. The high- and middle-dose Taohong Siwu Decoction groups had significantly increased serum levels of VEGF and iNOS (P<0.05) and a significantly reduced serum level of ET-1 (P<0.05). Conclusion Taohong Siwu Decoction can promote uterine repair in rats with postpartum blood stasis, possibly by regulating uterine vasomotion and promoting uterine angiogenesis.

关 键 词：桃红四物汤 祛瘀生新 促血管生成因子 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]