高密度脂蛋白模拟肽修复肿瘤坏死因子α诱导的内皮祖细胞损伤  

High density lipoprotein mimic peptide repairs the damage of endothelial progenitor cells functions induced by tumor necrosis factor α

作　　者：王凤娇 高翔[2] 孔淑琦 张建林[3] 刘祺 尹航 王梦洁 王晓丹[2] 牟青杰 杨娜娜 WANG Feng-Jiao;GAO Xiang;KONG Shu-Qi(Experimental Center for Medical Research,Weifang Medical University,Weifang 261000,Shandong,China)

机构地区：[1](1潍坊医学院医学研究实验中心,山东潍坊261000 [2]泰山医学院 [3]泰安市中心医院

出　　处：《中国老年学杂志》2019年第15期3729-3731,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81600360);山东省科技发展计划(2014GSF118105);山东省高等学校科技计划项目(J14LK03,J8KA272)

摘　　要：目的观察高密度脂蛋白模拟肽(Rev-D4F)对肿瘤坏死因子(TNF)-α诱导的内皮祖细胞凋亡和黏附的影响。方法差速贴壁法培养内皮祖细胞;吸附低度脂蛋白和结合凝集素方法鉴定内皮祖细胞;分别给予Rev-D4F(50μg/ml)和TNF-α(30ng/ml)进行处理,采用黏附测定实验和流式检测内皮祖细胞的黏附功能和凋亡情况。结果TNF-α促进内皮祖细胞凋亡,但TNF-α对内皮祖细胞黏附并没有作用,Rev-D4F预先干预能明显抑制TNF-α诱导的内皮祖细胞凋亡,提高内皮祖细胞黏附功能。结论Rev-D4F抑制TNF-α诱导的内皮祖细胞凋亡,提高内皮祖细胞黏附功能。Objective To study the effects of high density lipoprotein mimic peptide Rev-D4F on the apoptosis and adhesion of endothelial progenitor cells(EPCs)induced by tumor necrosis factor(TNF)-α.Methods EPCs were cultured by differential time attachment and identified by adsorption of low lipoprotein and binding lectin.EPCs were treated with Rev-D4F(50μg/ml)and TNF-α(30 ng/ml)respectively,the adhesion function and apoptosis of EPCs were measured by adhesion assay and flow cytometry.Results TNF-αpromoted EPCs apoptosis,but had no effect on EPCs adhesion.Rev-D4F pretreatment significantly inhibited TNF-αinduced EPCs apoptosis and improved endothelial progenitor cell adhesion function.Conclusions Rev-D4F could inhibit the apoptosis of EPCs induced by TNF-αand improve the adhesion function of EPCs.

关 键 词：高密度脂蛋白 肿瘤坏死因子 内皮祖细胞 

分 类 号：R28[医药卫生—中药学]