抗环瓜氨酸化抗体分离及其对巨噬细胞功能影响  

Anti-citrullinated protein antibodies(ACPAs)isolation and ACPAs-induced macrophage activity

作　　者：任丹丹 朱晓冬[1] 邱收 于鑫 朱伟[1] REN Dandan;ZHU Xiaodong;QIU Shou;YU Xin;ZHU Wei(Department of Immunology,Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院免疫教研室

出　　处：《生物加工过程》2019年第4期347-350,共4页Chinese Journal of Bioprocess Engineering

基　　金：黑龙江省自然科学基金(H2016093)

摘　　要：从类风湿性关节炎(RA)患者血清中分离抗环瓜氨酸化抗体(ACPAs),观察ACPAs对巨噬细胞功能的影响,探讨ACPAs影响RA病情发展的可能机制。利用亲和层析从RA患者血清中分离ACPAs,通过荧光定量PCR、蛋白印迹(Western blotting)及流式细胞术等实验方法观察ACPAs对巨噬细胞功能的影响。结果表明:亲和层析可很好地从血清中分离ACPA。ACPAs(40000IU/L)可促进巨噬细胞肿瘤坏死因子α(TNFα)、白细胞介素6(IL-6)、诱导型一氧化氮合成酶(iNOS)、人类白细胞DR抗原(HLA-DR)、磷酸化蛋白激酶B(p-AKT)等炎性因子的表达。SiRNA转染实验证实:ACPAs通过促进AKT蛋白磷酸化,诱导TNFα、IL6、iNOS的表达。Anti-citrullinated protein antibodies(ACPAs)were isolated from rheumatoid arthritis(RA)patient,and the mechanism of ACPAs-induced macrophage activities was studied.ACPAs were purified by affinity column.Fluorescence-activated cell sorting,Western blotting,and real-time PCR analyses were performed to detect the ACPAs-induced macrophage action.Results show that ACPAs(40 000 IU/L)could induce tumor necrosis factor α(TNFα),interleukin(IL)-6,inducible nitric oxide synthase(iNOS),human leukocyte antigen(HLA)-DR and phosphorylated protein kinase B(p-AKT)expression in macrophage.ACPAs increased TNFα,IL-6 and iNOS expression through promoting p-AKT expression.

关 键 词：抗环瓜氨酸化抗体 巨噬细胞 蛋白激酶B 类风湿性关节炎 

分 类 号：R392.9[医药卫生—免疫学]